Evidence map›Paper›PMID 40964071›Full record

ArticleNature photonics2025

Single molecule orientation and localization microscopy.

Sophie Brasselet, Matthew D Lew

Abstract read
In one paragraph

Article in Nature photonics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
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  8. Roadmap on singular optics and its applications.Applied physics. B, Lasers and optics · 2026
    Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Sophie BrasseletAix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.
Matthew D LewPreston M. Green Department of Electrical and Systems Engineering, Washington University in St. Louis, MO, USA.

Funding

Six-Dimensional Single-Molecule Nanoscopy for Elucidating the Dynamic Organization of BiomoleculesR35GM124858 · NIGMS · WASHINGTON UNIVERSITY · PI Matthew D Lew · 2017 to 2026
$3.3M
NIGMS NIH HHS R35 GM124858
6 · The paper itself

Abstract

Single Molecule Localization Microscopy (SMLM) offers enhanced spatial resolution in optical microscopy, providing detailed insights into the spatial organization of proteins in cells at the nanoscale. Over the past decade, SMLM has progressively incorporated the capability to retrieve the orientations of single molecules using their polarized dipolar emission pattern. This Review explores recent advancements in Single Molecule Orientation and Localization Microscopy (SMOLM), which yields super-resolved images of molecular 3D orientations, wobble, and 3D positions. This advancement opens possibilities to explore the nanoscale organization and conformation of biological molecules as well as to monitor and design local 3D optical fields in nanophotonics. The Review covers the principles of SMOLM, discusses recent advances and applications in biology and photonics, and finally highlights exciting future directions and challenges in the field.

Identifiers

PMID40964071
PMCPMC12440149

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.