Evidence map›Paper›PMID 40965357›Full record

ArticleEpilepsia2026

Extracellular vesicle microRNAs are biomarkers of focal epilepsy but not epilepsy-related respiratory dysfunction.

Sylvain Rheims, Hayet Kouchi, Florence Busato, Stanislas Lagarde, David Derbala, Sébastien Boulogne, Mathilde Leclercq, Jessica Chenais, Sandrine Bouvard, Fabrice Bartolomei and 2 more

Abstract read
In one paragraph

Article in Epilepsia, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Sylvain RheimsDepartment of Functional Neurology and Epileptology, Hospices Civils de Lyon and Lyon 1 University, Lyon, France.ORCID https://orcid.org/0000-0002-4663-8515
Hayet KouchiLyon Neuroscience Research Center, INSERM U1028/CNRS UMR 5292/Lyon 1 University, Lyon, France.ORCID https://orcid.org/0000-0003-1443-0645
Florence BusatoLaboratory for Epigenetics & Environment, Centre National de Recherche en Génomique Humaine, CEA-Institut de Biologie François Jacob, Université Paris-Saclay, Evry, France.
Stanislas LagardeEpileptology and Cerebral Rhythmology, Assistance-Publique Hôpitaux de Marseille, Timone Hospital, Marseille, France.ORCID https://orcid.org/0000-0003-2916-1302
David DerbalaLaboratory for Epigenetics & Environment, Centre National de Recherche en Génomique Humaine, CEA-Institut de Biologie François Jacob, Université Paris-Saclay, Evry, France.
Sébastien BoulogneDepartment of Functional Neurology and Epileptology, Hospices Civils de Lyon and Lyon 1 University, Lyon, France.ORCID https://orcid.org/0000-0001-8681-7369
Mathilde LeclercqDepartment of Functional Neurology and Epileptology, Hospices Civils de Lyon and Lyon 1 University, Lyon, France.
Jessica ChenaisLaboratory for Epigenetics & Environment, Centre National de Recherche en Génomique Humaine, CEA-Institut de Biologie François Jacob, Université Paris-Saclay, Evry, France.
Sandrine BouvardLyon Neuroscience Research Center, INSERM U1028/CNRS UMR 5292/Lyon 1 University, Lyon, France.
Fabrice BartolomeiEpileptology and Cerebral Rhythmology, Assistance-Publique Hôpitaux de Marseille, Timone Hospital, Marseille, France.ORCID https://orcid.org/0000-0002-1678-0297
Laurent BezinLyon Neuroscience Research Center, INSERM U1028/CNRS UMR 5292/Lyon 1 University, Lyon, France.ORCID https://orcid.org/0000-0001-6411-4267
Jorg TostLaboratory for Epigenetics & Environment, Centre National de Recherche en Génomique Humaine, CEA-Institut de Biologie François Jacob, Université Paris-Saclay, Evry, France.

Funding

European Research Council ERC-2023-COG-101125118 (EPIAROUSAL).French Chapter of ILAE (Ligue Francaise contre l'Epilesie) 2019French Foundation for epilepsy research 2018 clinical research grantInstitut National de la Santé et de la Recherche Médicale Contrat d'interface Hospitalier 2018
6 · The paper itself

Abstract

objectiveThis study was undertaken to evaluate the diagnostic value of a set of preselected candidate microRNAs (miRNAs) extracted from plasma-based extracellular vesicles (EVs) to identify patients with seizure-related respiratory dysfunction.

methodsA two-step design was applied. Step 1 entailed selection of the relevant miRNAs based on the combination of a literature review and an exploratory study in epileptic rats with or without interictal respiratory dysfunction. Step 2 involved evaluation of the diagnostic value of this preselected panel of circulating exosomal miRNAs in a case-control study conducted in 25 healthy subjects and 50 patients with drug-resistant focal epilepsy undergoing video-electroencephalographic (EEG) monitoring. Based on video-EEG data, patients were separated into two groups: those with ictal/postictal hypoxemia (PIH; n = 24) and those without (noPIH; n = 26). Blood samples were collected in the interictal period (>24 h after the last seizure). Expression level of each miRNAs in EVs was compared (1) between all patients with epilepsy and controls and (2) between PIH and noPIH. Receiver operating characteristic (ROC) curves were generated, and the area under the curve (AUC) was calculated.

resultsFollowing Step 1, the final set of miRNAs selected for evaluation in the case-control study included 24 miRNAs, with nine selected from published data in patients because of their potential regulatory role in the serotoninergic pathway, brain response to hypoxia, or epilepsy and 15 selected from the preclinical study in epileptic rats. Three miRNAs significantly differed between patients with epilepsy and controls (ROC curve AUC: hsa-miR-22-3p, .74 [95% confidence interval (CI) = .63-.85]; hsa-miR-106b-5p, .69 [95% CI = .57-.82]; and hsa-miR-26a-5p, .72 [95% CI = .58-.85]). Only a trend toward higher expression levels was observed for hsa-miR-140-3p in PIH compared to noPIH (+5%, p = .064). SIGNIFICANCE: Whereas three miRNAs were robustly associated with epilepsy, none was significantly associated with seizure-related respiratory dysfunction. Additional studies are required, including analysis of the expression of plasmatic cell-free miRNAs, especially the miRNAs associated with interictal respiratory dysfunction in epileptic rats.

Indexed as

Epilepsies, PartialExtracellular VesiclesMicroRNAsRespiration DisordersAdolescentAdultAnimalsBiomarkersCase-Control StudiesElectroencephalographyFemaleHumansMaleMiddle AgedRatsYoung AdultBiomarkersMicroRNAscirculating miRNAepilepsypostictal hypoxemiaserotoninSUDEP

Identifiers

PMID40965357
PMCPMC12893299

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.