Evidence mapPaperPMID 40965406Full record

ArticleInvestigative ophthalmology & visual science2025

Systemic and Local Lipids in Nonhuman Primates With Drusen and Age-Related Maculopathies.

Carol Villafuerte-Trisolini, Sophie M Le, Tzu-Ni Sin, Leon Huynh, Megan Gong, Tony Shen, Jaewon Heo, Karolina Roszak, Ana Santos Raposo, James Graham and 5 more

Abstract read
In one paragraph

Article in Investigative ophthalmology & visual science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Aging of Nonhuman Primate Eyes Is Sexually Dimorphic.Investigative ophthalmology & visual science · 2026
    Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Carol Villafuerte-TrisoliniDepartment of Ophthalmology & Vision Science, University of California, Davis, Davis, California, United States.
Sophie M LeDepartment of Ophthalmology & Vision Science, University of California, Davis, Davis, California, United States.
Tzu-Ni SinDepartment of Ophthalmology & Vision Science, University of California, Davis, Davis, California, United States.
Leon HuynhDepartment of Ophthalmology & Vision Science, University of California, Davis, Davis, California, United States.
Megan GongDepartment of Ophthalmology & Vision Science, University of California, Davis, Davis, California, United States.
Tony ShenDepartment of Ophthalmology & Vision Science, University of California, Davis, Davis, California, United States.
Jaewon HeoDepartment of Ophthalmology & Vision Science, University of California, Davis, Davis, California, United States.
Karolina RoszakDepartment of Surgical & Radiological Sciences, School of Veterinary Medicine, University of California, Davis, Davis, California, United States.
Ana Santos RaposoDepartment of Surgical & Radiological Sciences, School of Veterinary Medicine, University of California, Davis, Davis, California, United States.
James GrahamCalifornia National Primate Research Center, Davis, California, United States.
Peter HavelCalifornia National Primate Research Center, Davis, California, United States.
Kevin ChoyDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, United States.
Sina FarsiuDepartment of Biomedical Engineering, Duke University, Durham, North Carolina, United States.
Sara M ThomasyDepartment of Ophthalmology & Vision Science, University of California, Davis, Davis, California, United States.
Glenn YiuDepartment of Ophthalmology & Vision Science, University of California, Davis, Davis, California, United States.

Funding

California National Primate Research CenterP51OD011107 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · 2023 to 2025
$23.7M
VISION RESEARCH CORE GRANTP30EY012576 · UNIVERSITY OF CALIFORNIA DAVIS · 1999 to 2025
$3.6M
VISION RESEARCHP30EY005722 · DUKE UNIVERSITY · 1985 to 2025
$2.7M
Soft drusen in rhesus macaques as a nonhuman primate model of early age-related macular degenerationR01EY032238 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · 2022 to 2025
$2.7M
NEI NIH HHS P30 EY005722NEI NIH HHS P30 EY012576NEI NIH HHS R01 EY032238NEI NIH HHS U24 EY029904NIH HHS P51 OD011107
6 · The paper itself

Abstract

Purpose: Lipids are a principal component of drusen and are involved in the pathobiology of age-related macular degeneration (AMD). Nonhuman primates (NHPs) develop macular drusen and may provide insight into circulating or local lipids in AMD. Methods: We evaluated aged rhesus macaques by fundus photography, optical coherence tomography (OCT), and fundus autofluorescence, as well as measured fasting plasma glucose, total cholesterol, high- and low-density lipoproteins, triglycerides, and apolipoprotein (Apo) A1, B, CIII, and E. Retinal tissues were collected for electron microscopy and immunostained for oil red O, ApoE, and ApoB. Results: Among 203 adult macaques (mean age 19.1 ± 3.1 years), 25 animals (12.1%) exhibited soft drusen with sub-RPE deposits, while 59 (28.6%) had yellow punctate dots that were mostly hyperautofluorescent without RPE elevation on OCT. Drusen prevalence increased with older age (P = 0.001) but not with plasma lipids (P > 0.05 for all), while the punctate dot phenotype was associated with older age (P = 0.014), higher fasting glucose (P = 0.023), low-density lipoprotein cholesterol (P = 0.022), and ApoB (P = 0.017). Ultrastructure revealed NHP drusen consisting of extracellular sub-RPE lipid particles, whereas punctate dots appeared to correspond to individual RPE cells with intracellular lipid vacuoles. Both sub-RPE and intra-RPE lipids of the two phenotypes contained neutral lipids and ApoE, while ApoE and ApoB appeared to be expressed in RPE. Conclusions: In rhesus macaques, soft drusen are extracellular lipid deposits associated with older age, while punctate dots are intracellular lipids linked to age, hyperglycemia, and hyperlipidemia, suggesting differential dysregulation of lipid transport in these NHP models of AMD.

Indexed as

LipidsMacular DegenerationRetinal DrusenAnimalsDisease Models, AnimalFemaleFluorescein AngiographyMacaca mulattaMaleRetinal Pigment EpitheliumTomography, Optical CoherenceLipids

Identifiers

PMID40965406
PMCPMC12449822

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.