Evidence map›Paper›PMID 40966021›Full record

ArticleScience progress

Identification and validation of mitochondrial ferroptosis and immune microenvironment-related hub biomarkers in liver cirrhosis by integrated bioinformatics analysis.

PengChao Deng, Ram Prasad Chaulagain, Babalola Deborah Oluwaseun, FeiYang Gao, JiaXin Wang, RanYan Gao, XinYu Jiang, FengChun Li, LingYi Xu, HaoXuan Xu and 2 more

Abstract read
In one paragraph

Article in Science progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

PengChao DengDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0009-0000-6125-5929
Ram Prasad ChaulagainDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0009-0003-8854-612X
Babalola Deborah OluwaseunDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0009-0004-9853-5149
FeiYang GaoDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0009-0007-8019-3656
JiaXin WangDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0009-0006-8727-7214
RanYan GaoDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0009-0001-0666-6490
XinYu JiangDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0009-0007-2762-9625
FengChun LiDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0009-0004-6540-6527
LingYi XuDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0009-0003-6496-3113
HaoXuan XuDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0009-0006-8893-9822
KaiXin YaoDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0009-0000-3979-6171
Shizhu JinDepartment of Gastroenterology and Hepatology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0000-0003-3613-0926

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BackgroundLiver cirrhosis represents a significant challenge to global public health. However, reliable biological markers for diagnosing liver cirrhosis are lacking in clinical practice.MethodsTranscriptome data from liver cirrhosis patients were acquired from the Gene Expression Omnibus database to identify coexpressed differentially expressed genes (DEGs). Mitochondria-related and ferroptosis-related genes were obtained from MitoCarta3.0 and FerrDB V2, respectively. Immune-related module genes were examined through Weighted Gene Co-Expression Network Analysis (WGCNA). By using WGCNA combined with machine learning methods, we identified immune-related biomarkers for liver cirrhosis. The immune cell infiltration was evaluated using CIBERSORTx, with core immune cell types further refined through LASSO regression and random forest. Hub biomarkers were validated using single-cell sequencing, with additional confirmation provided by histological staining and immunohistochemistry (IHC).ResultsThis study identified 2474 DEGs between liver cirrhosis and control groups. Intersection analysis with ferroptosis-related genes and mitochondria-related genes narrowed to 13 hub genes, from which machine learning selected 8 biomarkers. CIBERSORT and Wilcoxon tests revealed notable variations in the 12 immune cell types across the different groups. The WGCNA identified immune-related genes, with four immune-related biomarkers (

Indexed as

Computational BiologyFerroptosisLiver CirrhosisMitochondriaBiomarkersGene Expression ProfilingGene Regulatory NetworksHumansMachine LearningTranscriptomeBiomarkersbiomarkersferroptosisimmune microenvironmentLiver cirrhosismitochondria

Identifiers

PMID40966021
PMCPMC12446859

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.