Evidence map›Paper›PMID 40972716›Full record

ArticleJournal of advanced research2026

Multi-omics elucidation of recombinant collagen-mediated modulation of mesenchymal stem cell functions.

Taishan Liu, Juanli Dang, Chenhui Zhu, Xiaoxuan Ma, Linlin Qu, Huan Lei, Daidi Fan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Taishan LiuEngineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710127, China; Xi'an Innovative R&D Platform for New Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710127, China.
Juanli DangEngineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710127, China; Xi'an Innovative R&D Platform for New Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Chenhui ZhuEngineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710127, China; Xi'an Innovative R&D Platform for New Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710127, China.
Xiaoxuan MaEngineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710127, China; Xi'an Innovative R&D Platform for New Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710127, China.
Linlin QuEngineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710127, China; Xi'an Giant Biotechnology Co., Ltd., Xi'an 710076, China; Xi'an Innovative R&D Platform for New Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710127, China. Electronic address: qulinlin@nwu.edu.cn.
Huan LeiEngineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710127, China; Xi'an Innovative R&D Platform for New Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710127, China. Electronic address: leihuan@nwu.edu.cn.
Daidi FanEngineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710127, China; Xi'an Innovative R&D Platform for New Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710127, China. Electronic address: fandaidi@nwu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionRegenerative medicine leverages the potential of mesenchymal stem cells (MSCs) and biomaterials to overcome the limitations of traditional organ transplantation. Collagen, a key extracellular matrix component, is widely used, yet its molecular interactions with MSCs remain insufficiently understood.

objectivesThis study investigates how recombinant collagens modulate MSC behavior and paracrine functions to inform the development of bioactive scaffolds for tissue regeneration.

methodsRecombinant type I and III collagens were biosynthesized using Pichia pastoris. Multi-omics analyses, including transcriptomics and proteomics, were conducted to evaluate the effects of these collagens on MSC gene expression, secretory profiles, and functional impacts on fibroblasts, endothelial cells, and macrophages.

resultsRecombinant collagens regulated key MSC pathways related to angiogenesis, wound healing, adhesion, cytoskeletal organization, and osteogenesis. Proteomic data further revealed enhanced secretion of cytokines and growth factors that influenced the behavior of surrounding stromal and immune cells.

conclusionRecombinant collagen, as a bioactive material, can remodel the functions of mesenchymal stem cells. Specifically, recombinant type I collagen primarily activates the FAK/RHOA/ROCK signaling pathway, while recombinant type III collagen activates the PI3K/Akt signaling pathway; in contrast, bovine-derived type I collagen mainly regulates glycolysis. These findings further support the potential of recombinant collagen in constructing regenerative medicine scaffolds with intelligent regulatory functions.

Indexed as

CollagenCollagen Type ICollagen Type IIIMesenchymal Stem CellsRecombinant ProteinsAnimalsHumansMultiomicsOsteogenesisProteomicsRegenerative MedicineSaccharomycetalesSignal TransductionTissue ScaffoldsCollagenCollagen Type ICollagen Type IIIRecombinant ProteinsMesenchymal stem cellMulti-omicsRecombinant collagenRegenerative medicine

Identifiers

PMID40972716
PMCPMC13227244

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.