ArticleScandinavian journal of immunology2025
Evaluation of Inhibitory Activity of Novel Monoclonal Antibodies Against Cat Allergen Fel d 7 and Their Application to Analyse Allergen Extracts.
Article in Scandinavian journal of immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Evaluation of Inhibitory Activity of Novel Monoclonal Antibodies Against Cat Allergen Fel d 7 and Their Application to Analyse Allergen Extracts.Scandinavian journal of immunology · 2025Article
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5 authors.
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Abstract
Current characterisation of cat dander/hair extracts used for allergy diagnosis or allergen-specific immunotherapy is mainly standardised towards the major allergen Fel d 1, while other allergens such as the lipocalin Fel d 7 are insufficiently characterised in such allergen products. This study aimed to produce recombinant Fel d 7 (rFel d 7) and murine IgG monoclonal antibodies (mAbs) specific to it for quantification in allergen extracts and assess the potential of the mAbs in inhibiting patients' IgE in functional assays. rFel d 7 was expressed in E. coli, purified by Ni-affinity and ion exchange chromatography, and physicochemically characterised by circular dichroism, Fourier-transform infrared spectroscopy, dynamic light scattering and mass spectrometry. Twenty hybridoma cell lines producing Fel d 7-specific mAbs were generated and sandwich ELISA was established for the quantitation of Fel d 7. Six different cat allergen extracts from different manufacturers and prepared from different sources were analysed and the concentration ranged from 0.02 μg/mg to 22.59 μg/mg. A mAb pool recognising non-overlapping epitopes inhibited the binding of human IgE-antigen complex formation (63.7% highest inhibition) and IgE-Fel d 7 cross-linking and consequent degranulation of basophilic cells (57.2% highest inhibition). We demonstrate the vast difference of Fel d 7 content in different cat allergen extracts, highlighting the necessity of improved standardisation of cat allergen extracts. The inhibition results showed that the analysed mAbs effectively inhibit rFel d 7 binding to human IgE, an assay we recommend for assessing the potency of allergen extracts as part of extract standardisation.
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