Evidence map›Paper›PMID 40975393›Full record

ArticleMolecular metabolism2025

Development of a genetically encoded melanocortin sensor for high sensitivity in vivo imaging.

Yoon Namkung, Tal Slutzki, Joao Pedroso, Xiaohong Liu, Paul V Sabatini, Maia V Kokoeva, Stéphane A Laporte

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular metabolism, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yoon NamkungDepartment of Medicine, Division of Endocrinology and Metabolism, Research Institute of the McGill University Health Center, McGill University, Montréal, QC H4A 3J1, Canada.
Tal SlutzkiDepartment of Medicine, Division of Endocrinology and Metabolism, Research Institute of the McGill University Health Center, McGill University, Montréal, QC H4A 3J1, Canada; Integrated Program in Neuroscience, McGill University, Montréal, QC H3A 2B4, Canada.
Joao PedrosoDepartment of Medicine, Division of Endocrinology and Metabolism, Research Institute of the McGill University Health Center, McGill University, Montréal, QC H4A 3J1, Canada.
Xiaohong LiuDepartment of Medicine, Division of Endocrinology and Metabolism, Research Institute of the McGill University Health Center, McGill University, Montréal, QC H4A 3J1, Canada.
Paul V SabatiniDepartment of Medicine, Division of Endocrinology and Metabolism, Research Institute of the McGill University Health Center, McGill University, Montréal, QC H4A 3J1, Canada; Integrated Program in Neuroscience, McGill University, Montréal, QC H3A 2B4, Canada.
Maia V KokoevaDepartment of Medicine, Division of Endocrinology and Metabolism, Research Institute of the McGill University Health Center, McGill University, Montréal, QC H4A 3J1, Canada; Integrated Program in Neuroscience, McGill University, Montréal, QC H3A 2B4, Canada. Electronic address: maia.kokoeva@mcgill.ca.
Stéphane A LaporteDepartment of Medicine, Division of Endocrinology and Metabolism, Research Institute of the McGill University Health Center, McGill University, Montréal, QC H4A 3J1, Canada; Department of Pharmacology and Therapeutics, McGill University, Montréal, QC H3G 1Y6, Canada. Electronic address: stephane.laporte@mcgill.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe central melanocortin system, composed of peptides derived from pro-opiomelanocortin (POMC) such as the melanocyte-stimulating hormones (α-, β-, γ-MSH) and melanocortin 4 receptors (MC4R), along with the agouti-related protein (AgRP), plays a pivotal role in controlling energy balance. To elucidate the dynamic role of α-MSH release in regulating appetite, specific, sensitive, and spatiotemporally resolved genetic sensors are required.

methodsThe melanocortin 1 receptor (MC1R) scaffold was leveraged for its robust plasma membrane expression, high affinity for melanocortins and low affinity for AgRP to design a α-MSH selective sensor for in vivo use. This was achieved by integrating circularly permuted green fluorescent protein (cpGFP) into the receptor, which we named Fluorescence Amplified Receptor sensor for Melanocortin (FLARE

resultsThe FLARE

conclusionsFLARE

Indexed as

MelanocortinsReceptor, Melanocortin, Type 1Agouti-Related Proteinalpha-MSHAnimalsGreen Fluorescent ProteinsHEK293 CellsHumansMaleMiceMice, Inbred C57BLReceptor, Melanocortin, Type 4Agouti-Related Proteinalpha-MSHGreen Fluorescent ProteinsMelanocortinsReceptor, Melanocortin, Type 1Receptor, Melanocortin, Type 4Central appetite regulationCircularly permuted green fluorescent protein sensorG protein-coupled receptorHypothalamusInvivo brain imaging and photometryMelanocortin receptors MC1 and MC4α-MSH

Identifiers

PMID40975393
PMCPMC12516568

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.