Evidence mapPaperPMID 40977662Full record

ArticleWorld journal of gastrointestinal oncology2025

Ginsenoside F4 inhibits colorectal cancer progression by boosting dendritic cell maturation and remodeling the tumor microenvironment.

Wei Xie, Xue-Jian Li, Yu-Sen Zhong, Jie Fang, Hui Qi, Meng Yang, Hua-Zhong Ying, Chen-Huan Yu

Abstract read
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Article in World journal of gastrointestinal oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Wei XieKey Laboratory of Experimental Animal and Safety Evaluation, Hangzhou Medical College, Hangzhou 310013, Zhejiang Province, China.
Xue-Jian LiKey Laboratory of Experimental Animal and Safety Evaluation, Hangzhou Medical College, Hangzhou 310013, Zhejiang Province, China.
Yu-Sen ZhongKey Laboratory of Experimental Animal and Safety Evaluation, Hangzhou Medical College, Hangzhou 310013, Zhejiang Province, China.
Jie FangKey Laboratory of Experimental Animal and Safety Evaluation, Hangzhou Medical College, Hangzhou 310013, Zhejiang Province, China.
Hui QiDepartment of Surgery, University of California, San Diego, CA 92037, United States.
Meng YangDepartment of Surgery, University of California, San Diego, CA 92037, United States.
Hua-Zhong YingKey Laboratory of Experimental Animal and Safety Evaluation, Hangzhou Medical College, Hangzhou 310013, Zhejiang Province, China.
Chen-Huan YuHangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, Zhejiang Province, China. yuchenhuan2002@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundImmunotherapy that employs dendritic cells (DCs) to activate the patient's immune system has emerged as a promising therapeutic strategy to combat cancer; however, effective targeting agents are still limited. Ginsenoside F4, as a rare ginsenoside found in

aimTo investigate the antitumor effect of Ginsenoside F4 and mechanism on the maturation of DCs in colorectal cancer (CRC).

methodsThe changes in mature DC markers and cytokines generated after DCs were exposed to F4 were assessed using flow cytometry and enzyme-linked immunosorbent assay, respectively. The viability of CRC CT26 cells co-cultured with T lymphocytes was monitored by cell counting kit-8 assay. Furthermore, the histopathological characteristics and immune cell infiltration in tumor tissues of CT26-bearing mice were analyzed by hematoxylin-eosin and immunofluorescent staining. The expressions of apoptosis-relative proteins were detected by western blot assay.

resultsTreatment with F4 promoted the maturation of DCs, elevated the expressions of cluster of differentiation (CD) 83 and CD86, increased the secretion of interleukin (IL)-2, IL-10, and IL-12 p70, and upregulated the expressions of phosphorylated phosphoinositide 3-kinase, phosphorylated protein kinase B, and nuclear factor kappa-B (NF-κB) phosphorylated p65 in DCs, which enhanced antigen-specific CD8+ T-cell responses. However, these benefits could be reversed by the sphingosine-1-phosphate 1 (S1PR1) inhibitor fingolimod hydrochloride. Furthermore, oral administration with F4 inhibited tumor growth and increased DC and CD8+ T-cell infiltration in the tumor tissues of CT26-bearing mice.

conclusionThe results demonstrated that F4 inhibited the growth of CRC by maturing DCs through activating S1PR1-mediated phosphoinositide 3-kinase/protein kinase B and NF-κB pathways, which triggered the antitumor effects of CD8+ T cells. Therefore, F4 could serve as an antitumor immunomodulator for CRC treatment.

Indexed as

Colorectal cancerCytotoxic T lymphocyteDendritic cellsGinsenosidesSphingosine-1-phosphate 1Tumor microenvironment

Identifiers

PMID40977662
PMCPMC12444327

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.