Mechanistic Insight for Improving Transglutaminase Production Through Combined HVCP and LiCl Mutagenesis in Streptomyces mobaraensis.

Tingting Chang, Ge Lian, Luyao Bian, Chong Zhang

Abstract read

In one paragraph

Article in Current microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

4 authors.

Tingting ChangLaboratory of Food Industrial Enzyme Technology, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

Ge LianLaboratory of Food Industrial Enzyme Technology, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

Luyao BianLaboratory of Food Industrial Enzyme Technology, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

Chong ZhangLaboratory of Food Industrial Enzyme Technology, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. zhangchong@njau.edu.cn.

Funding

Taixing Dongsheng Biotechnology Co., Ltd HM0055

6 · The paper itself

Abstract

Transglutaminase (TGase) is widely used in food, medicine, textile, and other industries owing to its excellent ability to promote protein cross-linking. The growing demand urgently requires the improvement of TGase production. Streptomyces mobaraensis FETSm1 was subjected to complex mutagenesis by high-voltage atmospheric cold plasma (HVCP) in combination with the chemically assisted mutagen lithium chloride (LiCl), and then a mutant FETSm2 with a 140% increase in TGase activity (12 U/mL) was obtained. FETSm2 had higher intracellular ATP content, which promotes the metabolism of the cells. The expression of TGase in FETSm2 was twofold higher than that of FETSm1, while enhanced activase activity promoted pro-TGase activation, contributing to the eventual increased TGase production. The application of the CCD-RSM and GA-ANN models further enhanced TGase production. FETSm2 could achieve a 17.64 U/mL TGase activity at the optimal ratio of the peptone, MgSO

Indexed as

Lithium ChlorideMutagenesisStreptomycesTransglutaminasesBacterial ProteinsCulture MediaFermentationBacterial ProteinsCulture MediaLithium ChlorideTransglutaminases

Identifiers

PMID40982131

DOI10.1007/s00284-025-04512-7

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.