Evidence map›Paper›PMID 40984022›Full record

ArticleCell proliferation2026

LCN2-ACOD1 Signalling Affects the Post-Injury Regeneration of Skeletal Muscle Through Mediating Ferroptosis.

Xiaojing Hao, Hongwei Shi, Di Wu, Rui Liang, Tong Zhao, Wen Sun, Yue Wang, Xiuju Yu, Xiaomao Luo, Yi Yan and 3 more

Abstract read
In one paragraph

Article in Cell proliferation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Xiaojing HaoCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, People's Republic of China.
Hongwei ShiCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, People's Republic of China.
Di WuCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, People's Republic of China.
Rui LiangCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, People's Republic of China.
Tong ZhaoCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, People's Republic of China.
Wen SunDepartment of Nephrology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Yue WangCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, People's Republic of China.
Xiuju YuCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, People's Republic of China.
Xiaomao LuoCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, People's Republic of China.
Yi YanCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, People's Republic of China.
Jiayin LuCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, People's Republic of China.ORCID https://orcid.org/0000-0001-7315-0729
Haidong WangCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, People's Republic of China.ORCID https://orcid.org/0000-0002-2762-0600
Juan WangDepartment of Nephrology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.ORCID https://orcid.org/0000-0001-9452-7900

Funding

Doctoral Research Initiation Program of Shanxi Agricultural University 2021BQ69National Natural Science Foundation of China 32102634National Natural Science Foundation of China 82170751Scientific Research Project of Shanxi Province Incentive Funds SXBYKY2022039Shanxi Provincial Basic Research Program 202103021223166s
6 · The paper itself

Abstract

The normal growth and development of skeletal muscle are crucial for the proper function of organisms. During myoblast development, cell death is a fundamental physiological process, and skeletal muscle damage involves various types of cell death, including ferroptosis. However, ferroptosis-related biomarkers in skeletal muscle damage remain unclear. This study aimed to investigate the mechanisms by which lipocalin-2 (LCN2), a key protein of iron metabolism, regulates skeletal muscle regeneration post damage by mediating ferroptosis. When the gastrocnemius muscle (GAS) of mice is acutely injured, LCN2 is significantly upregulated early in the injury. In vitro, LCN2 participates in the inhibition of proliferation and differentiation of C2C12 cells via erastin-induced ferroptosis. Transcriptomic analysis after the overexpression of LCN2 revealed that the one with the most significant difference among all of the differentially expressed genes (DEGs) was aconitate decarboxylase 1 (Acod1). The inhibition of myogenic factors' expression by LCN2 was associated with the activation of the ferroptosis signalling pathway, partly attributed to the mitochondrial dysfunction. The ACOD1 inhibitor attenuated mitochondria-associated ferroptosis induced by LCN2 and alleviated the inhibitory effect of LCN2 on cell viability. These findings highlight the therapeutic potential of targeting the LCN2-ACOD1 signalling to promote myogenesis, providing promising strategies for facilitating the regeneration of skeletal muscle after injury and the treatment of muscle-related diseases.

Indexed as

FerroptosisLipocalin-2Muscle, SkeletalRegenerationAnimalsCell DifferentiationCell LineCell ProliferationMaleMiceMice, Inbred C57BLMuscle DevelopmentSignal TransductionLcn2 protein, mouseLipocalin-2ACOD1ferroptosisLCN2mitochondriaskeletal muscle regeneration

Identifiers

PMID40984022
PMCPMC13051941

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.