Evidence map›Paper›PMID 40984643›Full record

ArticleCNS neuroscience & therapeutics2025

Genetic Modification of Mesenchymal Stem Cell to Overexpress CXCR4 Enhances Treatment Efficacy for Brain Injury After Cardiopulmonary Resuscitation.

Yongfei Liu, Li Zhang, Jingxiang Wang, Yuan Qin, Liang Zhang, Anlin Yue, Zhongting Wang, Xiao Xiao, Shuang Wang, Lu Huang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in CNS neuroscience & therapeutics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yongfei LiuDepartment of Anesthesiology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.ORCID 0000-0002-7134-0825
Li ZhangDepartment of Anesthesiology, Xi'an No. 3 Hospital, the Affiliated Hospital of Northwest University, Xi'an, Shaanxi, China.
Jingxiang WangDepartment of Anesthesiology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Yuan QinDepartment of Anesthesiology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Liang ZhangDepartment of Anesthesiology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Anlin YueDepartment of Anesthesiology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Zhongting WangDepartment of Anesthesiology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Xiao XiaoDepartment of Anesthesiology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Shuang WangDepartment of Anesthesiology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Lu HuangDepartment of Anesthesiology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Changjun GaoDepartment of Anesthesiology, Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi, China.

Funding

Natural Science Basic Research Program of Shaanxi Province (No. 2023-JC-ZD-52) 2023-JC-ZD-52
6 · The paper itself

Abstract

aimTo investigate whether genetically modifying human umbilical cord-derived mesenchymal stem cells (MSC) to overexpress the CXCR4 receptor can enhance their therapeutic efficacy for treating brain injury following cardiac arrest (CA).

methodsMSC were engineered to overexpress CXCR4 (CXCR4-MSC) via lentiviral transduction. The migration capacity of these cells was tested using in vitro chemotaxis assays. In a rat model of CA/CPR, the homing ability of CXCR4-MSC to the brain was tracked in vivo, and their therapeutic effects on neuronal death and neurological recovery were assessed. The role of exosomes and their impact on key proteins (NLRP3, ASC, GSDMD) in the pyroptosis pathway was also investigated.

resultCXCR4 overexpression significantly enhanced the migration of MSC in vitro and their homing to injured brain tissue in vivo. Treatment with CXCR4-MSC markedly reduced neuronal death and improved neurological recovery in resuscitated rats. This was accompanied by decreased expression of NLRP3. Furthermore, exosomes derived from CXCR4-MSC were found to suppress pyroptosis-related proteins (NLRP3/ASC/GSDMD) in post-CPR neurons, an effect that was reversed upon exosome inhibition.

conclusionGenetic modification to overexpress CXCR4 enhances the therapeutic efficacy of MSC for CA-induced brain injury by promoting their migration to the brain via the CXCL12/CXCR4 axis. A key mechanism of this protection is exosome-mediated inhibition of neuronal pyroptosis.

Indexed as

Brain InjuriesCardiopulmonary ResuscitationMesenchymal Stem CellsMesenchymal Stem Cell TransplantationReceptors, CXCR4AnimalsCell MovementExosomesHumansMaleNLR Family, Pyrin Domain-Containing 3 ProteinRatsRats, Sprague-DawleyTreatment OutcomeCXCR4 protein, humanCxcr4 protein, ratNLR Family, Pyrin Domain-Containing 3 ProteinReceptors, CXCR4asphyxial cardiac arrestCXCR4exosomesmesenchymal stem cellpyroptosis

Identifiers

PMID40984643
PMCPMC12454672

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.