Evidence mapPaperPMID 40986355Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

The multimodal transcriptional response of denervated skeletal muscle involves regulation of

Cristofer Calvo, Casey O Swoboda, Fabian Montecino-Morales, Siddhant Nagar, Michael J Petrany, Chengyi Sun, Hima Bindu Durumutla, Mattia Quattrocelli, Douglas P Millay

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Muscle fibre denervation in ageing.Clinical science (London, England : 1979) · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Cristofer CalvoDivision of Molecular Cardiovascular Biology, Cincinnati Children's Hospital, Cincinnati, OH 45229.ORCID 0009-0000-6371-2089
Casey O Swoboda *Division of Molecular Cardiovascular Biology, Cincinnati Children's Hospital, Cincinnati, OH 45229.
Fabian Montecino-Morales *Division of Molecular Cardiovascular Biology, Cincinnati Children's Hospital, Cincinnati, OH 45229.ORCID 0000-0001-5775-3730
Siddhant NagarDivision of Molecular Cardiovascular Biology, Cincinnati Children's Hospital, Cincinnati, OH 45229.
Michael J PetranyDivision of Molecular Cardiovascular Biology, Cincinnati Children's Hospital, Cincinnati, OH 45229.
Chengyi SunDivision of Molecular Cardiovascular Biology, Cincinnati Children's Hospital, Cincinnati, OH 45229.ORCID 0000-0001-8500-1878
Hima Bindu DurumutlaDivision of Molecular Cardiovascular Biology, Cincinnati Children's Hospital, Cincinnati, OH 45229.
Mattia QuattrocelliDivision of Molecular Cardiovascular Biology, Cincinnati Children's Hospital, Cincinnati, OH 45229.ORCID 0000-0001-7011-1740
Douglas P MillayDivision of Molecular Cardiovascular Biology, Cincinnati Children's Hospital, Cincinnati, OH 45229.ORCID 0000-0001-5188-0720

Funding

Myonuclear dynamics during skeletal muscle agingR01AG082697 · CINCINNATI CHILDRENS HOSP MED CTR · 2025 to 2025
$415k
Harnessing novel glucocorticoid biology to treat diabetic cardiomyopathyR01HL166356 · CINCINNATI CHILDRENS HOSP MED CTR · 2025 to 2025
$401k
Coordinated mechanisms to rescue bioenergetics and sarcopenia in agingR01AG078174 · CINCINNATI CHILDRENS HOSP MED CTR · 2025 to 2025
$319k
HHS | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) R01AR068286HHS | NIH | National Institute on Aging (NIA) R01AG082697NHLBI NIH HHS R01 HL166356NIAMS NIH HHS R01 AR068286NIA NIH HHS R01 AG078174NIA NIH HHS R01 AG082697NIDDK NIH HHS R03 DK130908
6 · The paper itself

Abstract

The development and maintenance of the neuromuscular junction (NMJ) requires reciprocal signals between the nerve terminals and multinucleated skeletal muscle fibers (myofibers). This interaction drives highly specialized transcription in the subsynaptic or NMJ myonuclei within mature myofibers leading to clustering of acetylcholine receptors (AChRs). Here, we utilized single-nucleus RNA sequencing (snRNA-seq) to delineate the transcriptional response of myonuclei to denervation. Through snRNA-seq on skeletal muscle from two independent mouse models of denervation, sciatic nerve transection and amyotrophic lateral sclerosis, we identify a multimodal transcriptional response of NMJ-enriched genes and an alteration in cholesterol homeostasis in myofibers.

Indexed as

Muscle, SkeletalAmyotrophic Lateral SclerosisAnimalsAutophagyCholesterolGene Expression RegulationMaleMiceMice, Inbred C57BLMuscle DenervationMuscle Fibers, SkeletalNeuromuscular JunctionReceptors, CholinergicSciatic NerveTranscription, GeneticCholesterolReceptors, CholinergiccholesterolGramd1b/Asterneuromuscular synapsesnRNA sequencing

Identifiers

PMID40986355
PMCPMC12501200

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.