Evidence map›Paper›PMID 40988045›Full record

ArticleJournal of animal science and biotechnology2025

Integrated mRNA-seq and miRNA-seq analysis reveals miR-210a-5p regulates uterine aging in laying hens by targeting the RASL11B/Raf/MAPK pathway.

Xiyu Zhao, Xinyan Li, Wenxin Zhang, Mingyue Gao, Conghao Zhong, Boxuan Zhang, Congjiao Sun, Yao Zhang, Shunshun Han, Huadong Yin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiyu Zhao *State Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, and, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology , Sichuan Agricultural University, 611130, Sichuan, People's Republic of China.
Xinyan Li *State Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, and, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology , Sichuan Agricultural University, 611130, Sichuan, People's Republic of China.
Wenxin ZhangState Key Laboratory of Animal Biotech Breeding, Frontier Science Center of Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Mingyue GaoState Key Laboratory of Animal Biotech Breeding, Frontier Science Center of Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Conghao ZhongState Key Laboratory of Animal Biotech Breeding, Frontier Science Center of Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Boxuan ZhangState Key Laboratory of Animal Biotech Breeding, Frontier Science Center of Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Congjiao SunState Key Laboratory of Animal Biotech Breeding, Frontier Science Center of Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Yao ZhangState Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, and, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology , Sichuan Agricultural University, 611130, Sichuan, People's Republic of China.
Shunshun HanState Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, and, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology , Sichuan Agricultural University, 611130, Sichuan, People's Republic of China. hanshunshun@sicau.edu.cn.
Huadong YinState Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, and, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology , Sichuan Agricultural University, 611130, Sichuan, People's Republic of China. yinhuadong@sicau.edu.cn.

Funding

China Agriculture Research System of MOF and MARA CARS-40Sichuan Province Science and Technology Support Program 2021YFYZ0031Sichuan Province Science and Technology Support Program 2022YFYZ0005Sichuan Province Science and Technology Support Program 2024YFNH0025The National Key Research and Development Program of China 2021YFD1300600
6 · The paper itself

Abstract

backgroundUterine aging is a key factor contributing to the deterioration of egg quality and reproductive performance in laying hens. Despite its importance, the molecular mechanisms underlying uterine aging remain poorly defined. This study aimed to characterize gene expression and regulatory changes associated with uterine aging in hens at different life stages.

resultsTranscriptomic Analysis of uterine tissue from hens aged 350, 500, And 700 d revealed dynamic changes in gene expression patterns during aging. A significant upregulation of genes involved in cellular senescence was observed, including increased expression of the p53 signaling pathway And markers associated with inflammation And cell cycle arrest. The most notable changes occurred between 350 And 500 d of age, suggesting this as a critical window for the onset of uterine aging. MicroRNA sequencing identified miR-210a-5p as significantly reduced with age. Target prediction and experimental validation showed that miR-210a-5p directly suppresses the expression of RASL11B, a Ras-like small GTPase that activates the MAPK signaling pathway. In primary uterine epithelial cells, reduced miR-210a-5p levels led to elevated RASL11B expression, increased activation of B-Raf, MEK, and ERK proteins, and enhanced expression of aging-related genes and inflammatory factors. In contrast, overexpression of miR-210a-5p or inhibition of the MAPK pathway delayed senescence and reduced inflammatory signaling. RASL11B overexpression was sufficient to induce aging phenotypes, confirming its central role in promoting uterine cellular aging.

conclusionsThis study identifies a novel regulatory pathway in which miR-210a-5p modulates uterine aging through the RASL11B-MAPK signaling cascade. The findings provide mechanistic insight into age-related reproductive decline in hens and suggest that targeting this pathway may offer new strategies for maintaining uterine function and extending reproductive lifespan in poultry.

Indexed as

Epithelial senescenceLaying henMAPK pathwayMiR-210a-5pRASL11BUterine aging

Identifiers

PMID40988045
PMCPMC12455797

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.