Evidence map›Paper›PMID 40990401›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

The Mitochondrial Protein RESISTANCE to APHIDS 9 Interacts with S40 to Resist Aphid Infestation by Modulating Reactive Oxygen Species Homeostasis in Maize (Zea mays).

Chuanhong Wang, Xinqiao Zhang, Zhen Tao, Lei Wang, Shijie Huang, Tengyue Wang, Yibing Zhao, Jinghui Dong, Jing Ma, Chang Chang and 3 more

Abstract read
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Achieving High-Density and Stress-Resilient Maize Breeding Via Germplasm Innovation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Chuanhong WangThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Xinqiao ZhangThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Zhen TaoThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Lei WangThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Shijie HuangThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Tengyue WangThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Yibing ZhaoThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Jinghui DongThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Jing MaThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Chang ChangThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Xingzhi ChenThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Ning LinThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
Peijin LiThe National Key Engineering Lab of Crop Stress Resistance Breeding, the School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.ORCID https://orcid.org/0000-0003-1579-7553

Funding

Anhui Provincial Key Research and Development Plan 202203a06020005National Key Research and Development Program of China 2022YFD1201803-2National Natural Science Foundation of China 32171954National Natural Science Foundation of China 32202322National Natural Science Foundation of China 32372084Scientific Research Project of the Anhui Provincial Department of Education 2023AH051054
6 · The paper itself

Abstract

As the corn aphid (Rhopalosiphum maidis) poses a major threat to maize (Zea mays) growth, there is much interest in identifying aphid resistance genes. In this study, an aphid-susceptible maize mutant from an ethyl methanesulfonate-mutagenized library is identified that exhibits greater aphid settlement than the wild type. Using the MutMap approach, the causal gene RESISTANCE TO APHIDS 9 (RTA9) is cloned, which encodes a mitochondrion-localized protein from the Domain of Unknown Function 641 family. Overexpressing RTA9 in maize confers significant resistance to aphids without compromising seed yield. It further identifies the senescence regulator S40 as an interactor of RTA9, which negatively regulates the stability of S40. Knockout of S40 enhanced aphid resistance, while its overexpression increased susceptibility. Further analysis demonstrates that the rta9-1 mutant does not exhibit significant enrichment of differentially expressed genes associated with oxidoreductase activity following aphid infestation. By contrast, genes involved in this pathway are significantly enriched in the s40 mutant. Additionally, aphid-induced reactive oxygen species (ROS) levels are markedly lower in rta9-1 than in the wild type but significantly higher in s40. Collectively, the results suggest that the mitochondrial protein RTA9 and its interacting partner S40 regulate resistance to aphid infestation by modulating ROS homeostasis.

Indexed as

AphidsDisease ResistanceMitochondrial ProteinsPlant DiseasesPlant ProteinsReactive Oxygen SpeciesZea maysAnimalsHomeostasisMitochondriaMitochondrial ProteinsPlant ProteinsReactive Oxygen Speciesaphids (Rhopalosiphum maidis)DUF641maize (Zea mays)mitochondrial proteinreactive oxygen species (ROS) homeostasisRTA9S40

Identifiers

PMID40990401
PMCPMC12697904

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.