Evidence mapPaperPMID 40993350Full record

ArticleNature nanotechnology2025

Meso-macroporous hydrogel for direct litre-scale isolation of extracellular vesicles.

Junbeom Kim, Minjin Kang, Geonhee Han, Sujin Hyung, Mina Kim, Minjeong Jang, Han Kyul Lee, Yunhee Seo, Ki Cheol Gil, Changheon Kim and 22 more

Abstract read
In one paragraph

Article in Nature nanotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

32 authors.

Junbeom KimBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Korea.ORCID http://orcid.org/0009-0005-6213-181X
Minjin KangBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Korea.ORCID http://orcid.org/0009-0007-1695-430X
Geonhee HanMedicinal Materials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST), Seoul, Korea.
Sujin HyungPrecision Medicine Research Institute, Samsung Medical Center, Seoul, Korea.ORCID http://orcid.org/0000-0003-1192-0972
Mina KimKU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Korea.ORCID http://orcid.org/0000-0002-2381-8011
Minjeong JangBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Korea.
Han Kyul LeeChemical & Biological Integrative Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST), Seoul, Korea.ORCID http://orcid.org/0009-0002-5371-0447
Yunhee SeoAdvanced Analysis and Data Center, Research Resources Division, Korea Institute of Science and Technology (KIST), Seoul, Korea.ORCID http://orcid.org/0000-0002-5721-2974
Ki Cheol GilMedicinal Materials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST), Seoul, Korea.
Changheon KimDepartment of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA.
Sojin SongBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Korea.
Seonghyeok JeongBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Korea.
Seongchan KimCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju, Korea.ORCID http://orcid.org/0000-0002-2104-6599
Min Soo KimBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Korea.ORCID http://orcid.org/0000-0003-1892-8061
Ji Sung ShimDepartment of Urology, College of Medicine, Korea University, Seoul, Korea.
Sung Gu KangDepartment of Urology, College of Medicine, Korea University, Seoul, Korea.
Young Chan LeeDepartment of Otolaryngology-Head and Neck Surgery, Kyung Hee University College of Medicine, Kyung Hee University Hospital at Gangdong, Seoul, Korea.
Seok ChungKU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Korea.ORCID http://orcid.org/0000-0002-1735-8338
Il-Joo ChoDepartment of Biomedical Sciences, College of Medicine, Korea University, Seoul, Korea.ORCID http://orcid.org/0000-0001-9016-6749
Tae Soup ShimDepartment of Chemical Engineering, Ajou University, Suwon, Korea.ORCID http://orcid.org/0000-0003-2414-1599
Kwang Hoon SongDepartment of Nano-Bioengineering, Incheon National University, Incheon, Korea.
Jouha MinDepartment of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA.ORCID http://orcid.org/0000-0002-6737-7254
Hyejeong SeongBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Korea.ORCID http://orcid.org/0000-0002-9877-296X
Kyungeun LeeAdvanced Analysis and Data Center, Research Resources Division, Korea Institute of Science and Technology (KIST), Seoul, Korea.
Jeeyun LeeDivision of Hematology-Oncology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Cheolju LeeChemical & Biological Integrative Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST), Seoul, Korea.
Hong Nam KimBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Korea.ORCID http://orcid.org/0000-0002-0329-0029
Hyojin LeeDivision of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Seoul, Korea.ORCID http://orcid.org/0000-0001-6367-9485
Sun Hwa KimMedicinal Materials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST), Seoul, Korea.
Ji Yoon KangBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Korea. jykang@kist.re.kr.ORCID http://orcid.org/0000-0003-4595-0512
Ki Wan BongDepartment of Chemical and Biological Engineering, Korea University, Seoul, Korea. bong98@korea.ac.kr.ORCID http://orcid.org/0000-0001-5026-0757
Nakwon ChoiBrain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Korea. nakwonchoi@korea.ac.kr.ORCID http://orcid.org/0000-0003-2993-9233

Funding

Korea Institute of Science and Technology (KIST) 2E33682Korea Institute of Science and Technology (KIST) 2E33712Korea Institute of Science and Technology (KIST) 2V09840-23-P024Ministry of Food and Drug Safety (MFDS) RS-2024-00331678Ministry of Food and Drug Safety (MFDS) RS-2024-00332024National Research Foundation of Korea (NRF) RS-2020-NR046282National Research Foundation of Korea (NRF) RS-2022-NR067280National Research Foundation of Korea (NRF) RS-2023-00207833National Research Foundation of Korea (NRF) RS-2023-00222838National Research Foundation of Korea (NRF) RS-2024-00346657National Research Foundation of Korea (NRF) RS-2024-00347828National Research Foundation of Korea (NRF) RS-2024-00395393National Research Foundation of Korea (NRF) RS-2024-00459155National Research Foundation of Korea (NRF) RS-2024-0440577
6 · The paper itself

Abstract

Extracellular vesicles are cell-originated lipid bilayer membrane vesicles that play vital roles in cell-to-cell communications. While extracellular vesicles hold substantial biomedical potential, conventional methodologies for isolating extracellular vesicles require elaborate preprocessing and, therefore, remain labour intensive and limited by throughput. To overcome these challenges, we present a facile fabrication route for generating a meso-macroporous hydrogel matrix with pores of ~400 nm for customizable extracellular vesicle isolation. By combining surface charge-selective capture of extracellular vesicles within the hydrogel matrix and their recovery by high ionic strength, we report direct extracellular vesicle isolation with a throughput range from microlitre to litre scales, without preprocessing, for various biofluids, including whole blood, plasma, ascites, saliva, urine, bovine milk and cell culture media. Furthermore, we demonstrate that the meso-macroporous hydrogel also serves as a solid-phase matrix for preserving extracellular vesicles for on-demand downstream analyses, making it applicable for therapeutics, cosmeceuticals and disease diagnostics.

Indexed as

Extracellular VesiclesHydrogelsAnimalsCattleHumansPorosityHydrogels

Identifiers

PMID40993350
PMCPMC12623240

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.