Evidence mapPaperPMID 41002353Full record

ReviewBiosensors2025

Decoding Molecular Network Dynamics in Cells: Advances in Multiplexed Live Imaging of Fluorescent Biosensors.

Qiaowen Chen, Yichu Xu, Jhen-Wei Wu, Jr-Ming Yang, Chuan-Hsiang Huang

Abstract readReview
In one paragraph

Review in Biosensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Qiaowen ChenDepartment of Applied Mathematics and Statistics, Johns Hopkins University, Baltimore, MD 21218, USA.ORCID 0009-0009-6857-7977
Yichu XuDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA.ORCID 0009-0002-5321-8284
Jhen-Wei WuDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA.
Jr-Ming YangDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA.ORCID 0000-0002-6296-8987
Chuan-Hsiang HuangDepartment of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA.ORCID 0000-0001-9993-8004

Funding

Tissue/Pathology/Immunology CoreP50CA098252 · JOHNS HOPKINS UNIVERSITY · 2003 to 2025
$10.1M
NCI NIH HHS P50 CA098252NIGMS NIH HHS R01 GM136711NIH HHS P50CA098252NIH HHS R01GM136711Sol Goldman Pancreatic Cancer Research Center NA
6 · The paper itself

Abstract

Genetically encoded fluorescent protein (FP)-based biosensors have revolutionized cell biology research by enabling real-time monitoring of molecular activities in live cells with exceptional spatial and temporal resolution. Multiplexed biosensing advances this capability by allowing the simultaneous tracking of multiple signaling pathways to uncover network interactions and dynamic coordination. However, challenges in spectral overlap limit broader implementation. Innovative strategies have been devised to address these challenges, including spectral separation through FP palette expansion and novel biosensor designs, temporal differentiation using photochromic or reversibly switching FPs, and spatial segregation of biosensors to specific subcellular regions or through cell barcoding techniques. Combining multiplexed biosensors with artificial intelligence-driven analysis holds great potential for uncovering cellular decision-making processes. Continued innovation in this field will deepen our understanding of molecular networks in cells, with implications for both fundamental biology and therapeutic development.

Indexed as

Biosensing TechniquesHumansLuminescent ProteinsSignal TransductionLuminescent Proteinsfluorescent biosensorsFRETlive cell imagingmultiplexingsignaling dynamicssignaling network

Identifiers

PMID41002353
PMCPMC12467888

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.