Evidence map›Paper›PMID 41002370›Full record

ArticleBiosensors2025

SERS-Based Immunoassay for α-Fetoprotein Biomarker Detection Using an Au-Ag Nanostars Platform.

Josué Ismael García-Ramírez, Marcos Luna-Cervantes, Irma Yadira Izaguirre-Hernández, Julián Hernández-Torres, Enrique Juárez-Aguilar, Pablo Thomas-Dupont, José María Remes-Troche, Luis Zamora-Peredo

Abstract read
In one paragraph

Article in Biosensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Josué Ismael García-RamírezCentro de Investigación en Micro y Nanotecnología, Universidad Veracruzana, Boca del Río 94294, Mexico.
Marcos Luna-CervantesCentro de Investigación en Micro y Nanotecnología, Universidad Veracruzana, Boca del Río 94294, Mexico.
Irma Yadira Izaguirre-HernándezFacultad de Bioanálisis, Universidad Veracruzana, Veracruz 91700, Mexico.ORCID 0009-0009-4766-6516
Julián Hernández-TorresCentro de Investigación en Micro y Nanotecnología, Universidad Veracruzana, Boca del Río 94294, Mexico.ORCID 0000-0002-3517-1323
Enrique Juárez-AguilarInstituto de Ciencias de la Salud, Universidad Veracruzana, Xalapa-Enriquez 91190, Mexico.ORCID 0000-0003-3010-4364
Pablo Thomas-DupontInstituto de Investigaciones Médico Biológicas, Universidad Veracruzana, Veracruz 91700, Mexico.ORCID 0000-0001-9950-2083
José María Remes-TrocheInstituto de Investigaciones Médico Biológicas, Universidad Veracruzana, Veracruz 91700, Mexico.
Luis Zamora-PeredoCentro de Investigación en Micro y Nanotecnología, Universidad Veracruzana, Boca del Río 94294, Mexico.ORCID 0000-0002-8101-4708

Funding

Secretaría de Ciencia, Humanidades, Tecnología e Innovación (Secihti) CF-2023-G-580
6 · The paper itself

Abstract

Spiky Au-Ag nanostars offer intense plasmonic enhancement due to their sharp-tipped morphology, enabling powerful surface-enhanced Raman scattering (SERS). Here, we report a liquid-phase SERS platform that addresses current limitations in cancer biomarker detection, such as low sensitivity and dependence on Raman reporters. Nanostar concentration was tuned by simple centrifugation (10, 30, and 60 min), and their SERS performance was evaluated using methylene blue (MB) and mercaptopropionic acid (MPA) as probe molecules. Signal intensity scaled with nanostar content, enabling sensitive detection. Optimized nanostars were functionalized with MPA, 1-Ethyl-3-(3-dimethylamino1-Ethyl-3-(3dimethylaminopropyl1) carbodiimide (EDC), and N-Hydroxy succinimide (NHS) for covalent attachment of monoclonal anti-α-fetoprotein antibodies (AFP-Ab), facilitating the detection of AFP antigens across 167-38 ng/mL (antibody) and 500-0 ng/mL (antigen) ranges. The limit of detection (LOD) for the antigens was determined to be 16.73 ng/mL. Unlike conventional SERS systems, this aqueous, surfactant-free platform exploits the intrinsic vibrational modes of AFP, enabling sensitive and rapid biomarker detection with strong potential for early cancer diagnostics.

Indexed as

alpha-FetoproteinsMetal NanoparticlesSpectrum Analysis, RamanBiomarkers, TumorBiosensing TechniquesGoldHumansImmunoassayLimit of DetectionSilveralpha-FetoproteinsBiomarkers, TumorGoldSilveralpha-fetoproteinAu-Ag nanostarscancer biosensorliquid-phase SERS

Identifiers

PMID41002370
PMCPMC12468422

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.