ArticleCell biochemistry and biophysics2026
In-silico and In-vitro Gene Expression Analysis on Docetaxel and Methotrexate-induced Cytotoxicity in Oral Cancer Cell Line.
Article in Cell biochemistry and biophysics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Unraveling the molecular landscape: an overview on gene expression, epigenetic alterations, and therapeutic challenges in head and neck squamous cell carcinoma.Molecular genetics and genomics : MGG · 2026Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oral cancer is a major health burden, with many patients presenting with late-stage disease and distant metastasis, contributing to higher morbidity and mortality. Surgery, chemotherapy, and radiotherapy are common treatment modalities for oral cancer. Chemotherapeutic drugs are effective in treating cancers; however, they also have various side effects that can affect patient survival rates. Docetaxel (DTX) and methotrexate (MTX) are used to treat multiple solid tumors. However, there are limited reports available on the efficacy of these drugs against oral cancer. Thus, in the present study, we investigated the effect of DTX and MTX as monotherapies on KB oral cancer cell lines. Initially, functional enrichment, gene ontology, and protein-protein interaction (PPI) analyses were performed to understand the key biological processes and interaction networks associated with DTX- and MTX-targeted genes in oral carcinoma. In vitro analyses were conducted in KB cells treated with different concentrations of DTX (1.5–50 nM) and MTX (25 to 250µM) to identify the effective IC50 concentrations using MTT assay. Next, RT-PCR analysis was performed to evaluate the expression of 29 major genes involved in oral cancer, and apoptotic protein levels were analyzed using western blotting. DTX and MTX treatment resulted in significant upregulation of apoptotic and tumor suppressor genes, together with downregulation of oncogenes, drug transporters, growth factors, cell cycle regulators, and signal transduction genes. Western blot data showed that there was an increase in caspases (3,7, and 9) during DTX and MTX treatment in oral cancer cells. Moreover, enhanced DNA fragmentation, reduced cell migration, and decreased colony-forming ability have been observed. Overall, our data suggest that DTX showed a significant cytotoxic effect against oral cancer cells compared to MTX and could be used as an effective chemotherapeutic drug against oral cancer in the near future; however, extensive molecular investigations are still warranted.
Indexed as
Identifiers
41004018What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.