Evidence mapPaperPMID 41005974Full record

ArticleRMD open2025

Decreased GPR55 expression links B-cell activation and vascular remodelling in atherosclerosis in patients with early rheumatoid arthritis.

Daniel Miranda-Prieto, Mercedes Alperi-López, Ángel I Pérez-Álvarez, Sara Alonso-Castro, Ana Suárez, Javier Rodríguez-Carrio

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Article in RMD open, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Daniel Miranda-PrietoDepartment of Functional Biology, Area of Immunology, University of Oviedo, Oviedo, Spain.
Mercedes Alperi-LópezDepartment of Metabolism, Instituto de Investigación Sanitaria del Principado de Asturias, Oviedo, Spain.
Ángel I Pérez-ÁlvarezDepartment of Neurology, Hospital Universitario Central de Asturias, Oviedo, Spain.
Sara Alonso-CastroDepartment of Rheumatology, Hospital Universitario Central de Asturias, Oviedo, Asturias, Spain.
Ana SuárezDepartment of Functional Biology, Area of Immunology, University of Oviedo, Oviedo, Spain rodriguezcjavier@uniovi.es anasua@uniovi.es.
Javier Rodríguez-CarrioDepartment of Functional Biology, Area of Immunology, University of Oviedo, Oviedo, Spain rodriguezcjavier@uniovi.es anasua@uniovi.es.ORCID 0000-0002-0011-5102

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveInflammation and repair responses may be involved in atherosclerosis in rheumatoid arthritis (RA), although mechanisms are unknown. GPR55, a cannabinoid receptor expressed in haematopoietic and stromal tissues, has been implicated in atherosclerosis in mouse models, but evidence in humans is lacking. Our aim was to evaluate GPR55 expression in leucocyte populations in RA and their potential role in atherosclerosis.

methodsGPR55 expression was quantified by flow cytometry in 63 treatment-naïve patients with RA, 11 individuals with arthralgia and 36 controls. Atherosclerosis was assessed by Doppler ultrasound. Cytokines were measured by immunoassays, and serum proteomics were performed by a high-throughput targeted panel. In vitro cultures were performed with mononuclear cells from healthy donors.

resultsDecreased GPR55 expression in B-cells and monocytes was found in RA, whereas no differences were observed in arthralgia. Public datasets validated these findings. B-cell GPR55 expression was unrelated to clinical features, risk factors and atherosclerosis in RA, but exhibited divergent associations with leucocyte populations. GPR55 expression was associated with proinflammatory cytokines, immunoglobulin and antibody levels, metabolomic markers of inflammation and proteomic signatures related to vascular remodelling and B-cell responses in RA. These associations were dependent on the atherosclerosis status. Lipopolysaccharide exposure in vitro decreased GPR55 expression in B-cells in a dose-dependent manner, which overlapped increasing CB86 expression.

conclusionsReduced GPR55 expression hallmarked B-cells and monocyte subsets in early RA. GPR55 expression was linked to B-cell activation-related pathways, presumably via T-cell independent mechanisms and vascular remodelling. GPR55 may be a novel hub between immune circuits and maladaptive responses in atherosclerosis.

Indexed as

Arthritis, RheumatoidAtherosclerosisB-LymphocytesLymphocyte ActivationReceptors, CannabinoidVascular RemodelingAdultAgedBiomarkersCytokinesFemaleHumansLeukocytes, MononuclearMaleMiddle AgedBiomarkersCytokinesGPR55 protein, humanReceptors, CannabinoidArthritis, RheumatoidAtherosclerosisBiomarkersB-LymphocytesInflammation

Identifiers

PMID41005974
PMCPMC12481376

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.