ArticleScientific reports2025
Enhancing developmental potential of vitrified in vitro matured bovine oocytes using extracellular vesicles from large follicles.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Extracellular Vesicles From Heat- and Oxidative-Stressed Granulosa Cells Improve Redox Balance and Embryo Development in Bovine Oocytes.Reproduction in domestic animals = Zuchthygiene · 2026Article
- One-step warming procedure improves embryonic development of vitrified mouse oocytes by mitigating mitochondrial damage.Journal of assisted reproduction and genetics · 2026Article
- Extracellular vesicles in bovine reproduction: their journey from gametogenesis to pregnancy.Frontiers in cell and developmental biology · 2026Review
- Vitrification of bovine oocytes and embryos: roadmaps to cryopreservation success.Animal reproduction · 2026Article
- Follicular fluid extracellular vesicles improve bovine oocyte quality via lipid and mitochondrial modulation.Frontiers in veterinary science · 2025Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
This study investigates the differential effects of extracellular vesicles (EVs) derived from bovine follicular fluid (FF) on bovine oocytes cryotolerance, focusing on EVs from small (3-5 mm) and large (> 9 mm) follicles, with the aim of optimizing vitrification protocols. FF-EVs were isolated and characterized for size and concentration. Oocytes were in vitro matured with FF-EVs prior to vitrification. Post-warming assessment included spindle morphology, DNA integrity, embryo development, total cell count, and apoptotic index of resulting blastocysts. EVs uptake by cumulus-oocyte complexes was confirmed via confocal microscopy. While percentages of oocytes at the metaphase II stage were similar across groups, vitrification significantly reduced normal spindle configurations, except in oocytes supplemented with large FF-EVs (VIT Large), which resembled fresh oocytes. DNA fragmentation was significantly lower in the VIT Large group, whereas vitrification alone increased fragmentation. Blastocyst, expansion and hatching rates were higher in VIT Large, which also preserved inner cell mass quality similar to fresh counterparts. These findings suggest that FF-EVs from large follicles mitigate vitrification-induced damage, improving oocyte cryopreservation outcomes and embryo quality. To the best of our knowledge, this is the first study to demonstrate that FF-EVs can improve the cryotolerance of bovine oocytes, establishing a novel approach for enhancing oocyte vitrification outcomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.