Evidence mapPaperPMID 41006534Full record

SynthesisScientific reports2025

In vitro and in silico studies and a systematic literature review of antiglycation properties of amlodipine.

Karolina Dańkowska, Miłosz Nesterowicz, Kamil Klaudiusz Lauko, Daria Trocka, Małgorzata Żendzian-Piotrowska, Jerzy Robert Ładny, Anna Zalewska, Marta Żebrowska-Gamdzyk, Mateusz Maciejczyk

Abstract readSystematic Review
In one paragraph

Synthesis in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Karolina DańkowskaStudents' Scientific Club "Biochemistry of Civilization Diseases Department of Hygiene, Epidemiology and Ergonomics , Medical University of Bialystok , 2c Mickiewicza Street, 15-233, Białystok, Poland.
Miłosz NesterowiczStudents' Scientific Club "Biochemistry of Civilization Diseases Department of Hygiene, Epidemiology and Ergonomics , Medical University of Bialystok , 2c Mickiewicza Street, 15-233, Białystok, Poland.
Kamil Klaudiusz LaukoStudents' Scientific Club "Biochemistry of Civilization Diseases Department of Hygiene, Epidemiology and Ergonomics , Medical University of Bialystok , 2c Mickiewicza Street, 15-233, Białystok, Poland.
Daria TrockaStudents' Scientific Club "Biochemistry of Civilization Diseases Department of Hygiene, Epidemiology and Ergonomics , Medical University of Bialystok , 2c Mickiewicza Street, 15-233, Białystok, Poland.
Małgorzata Żendzian-PiotrowskaDepartment of Hygiene, Epidemiology and Ergonomics , Medical University of Bialystok , Białystok, 2c Mickiewicza Street, 15-233, Poland.
Jerzy Robert ŁadnyDepartment of Emergeny Medicine , Medical University of Bialystok , Białystok, 24a M. Sklodowskiej-Curie Street, 15-274, Poland.
Anna ZalewskaIndependent Laboratory of Experimental Dentistry , Medical University of Bialystok , Białystok, 24a M. Sklodowskiej-Curie Street, 15-274, Poland.
Marta Żebrowska-GamdzykDepartment of Dietetics Faculty of Health Sciences, Łomża Academy, Łomża, 14 Akademicka Street, 18-400, Poland.
Mateusz MaciejczykDepartment of Hygiene, Epidemiology and Ergonomics , Medical University of Bialystok , Białystok, 2c Mickiewicza Street, 15-233, Poland. mat.maciejczyk@gmail.com.

Funding

Medical Unversity of Bialystok B.SUB.24.250
6 · The paper itself

Abstract

Protein glycation is crucial in the pathogenesis of diabetes and its cardiovascular complications. Little is known about the antiglycation properties of amlodipine, a long-acting calcium channel blocker used to treat high blood pressure. In our study, amlodipine's antiglycoxidant activity was assayed in sugars (glucose, fructose, and ribose), aldehydes (glyoxal), and chloramine T-modified bovine serum albumin (BSA). Aminoguanidine and N-acetylcysteine were used as standard glycation/oxidation inhibitors. The content of oxidation, glycoxidation, and glycation protein products was measured colorimetrically and fluorimetrically. A one-way analysis of variance (ANOVA) followed by Tukey's post hoc test was used for statistical analysis. The mechanism of amlodipine's antiglycoxidant activity was also evaluated using in-silico molecular docking. Amlodipine protects against BSA oxidation, as evidenced by enhanced total thiol content and mitigated protein carbonyls/advanced oxidation protein products. Amlodipine also increased the fluorescence of tryptophan and decreased the contents of kynurenine, N-formylkynurenine, and dityrosine. In addition, amlodipine effectively prevents protein glycation, as evidenced by a reduction in amyloid-beta structure, Amadori products, and advanced glycation end products (AGEs). In in silico analysis, amlodipine's antiglycation properties were indicated during its interaction with BSA, glycosidases, and AGEs/receptor for AGEs (RAGE) pathway proteins. Among all proteins, amlodipine docked best with c-Jun N-terminal kinases. Summarizing, we have demonstrated the anti-glycation and antioxidant activity of amlodipine in vitro. This effect may be particularly important in patients with diabetes and atherosclerosis, where excessive glycation accelerates the development of vascular complications. Further studies are needed to confirm the antidiabetic activity of amlodipine in vivo.

Indexed as

AmlodipineAnimalsComputer SimulationGlycation End Products, AdvancedGlycosylationHumansMolecular Docking SimulationOxidation-ReductionSerum Albumin, BovineAmlodipineGlycation End Products, AdvancedSerum Albumin, Bovine

Identifiers

PMID41006534
PMCPMC12474885

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.