Evidence mapPaperPMID 41006917Full record

ArticleNature cardiovascular research2025

Quantitative proteomics of formalin-fixed, paraffin-embedded cardiac specimens uncovers protein signatures of specialized regions and patient groups.

Jonathan S Achter, Thomas H L Jensen, Paola Pisano, Johan S Bundgaard, Daniel Raaschou-Oddershede, Kasper Rossing, Michael Wierer, Alicia Lundby

Abstract read
In one paragraph

Article in Nature cardiovascular research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jonathan S AchterDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.ORCID http://orcid.org/0000-0002-1587-622X
Thomas H L JensenDepartment of Pathology, Diagnostic Center, Copenhagen University Hospital, Copenhagen, Denmark.
Paola PisanoProteomics Research Infrastructure, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Johan S BundgaardDepartment of Cardiology, The Heart Center, Copenhagen University Hospital, Copenhagen, Denmark.ORCID http://orcid.org/0000-0003-2069-9034
Daniel Raaschou-OddershedeDepartment of Cardiology, The Heart Center, Copenhagen University Hospital, Copenhagen, Denmark.ORCID http://orcid.org/0000-0002-0591-3656
Kasper RossingDepartment of Cardiology, The Heart Center, Copenhagen University Hospital, Copenhagen, Denmark.ORCID http://orcid.org/0000-0002-5253-1247
Michael WiererProteomics Research Infrastructure, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Alicia LundbyDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. alicia.lundby@sund.ku.dk.ORCID http://orcid.org/0000-0002-1612-6041

Funding

Det Frie Forskningsråd (Danish Council for Independent Research) https://doi.org/10.46540/4285-00115BNovo Nordisk Fonden (Novo Nordisk Foundation) NNF20OC0059767
6 · The paper itself

Abstract

Proteomic technologies have advanced our understanding of disease mechanisms, patient stratification and targeted therapies. However, applying cardiac proteomics in translational research requires overcoming the barrier of tissue accessibility. Formalin-fixed, paraffin-embedded (FFPE) heart tissue, widely preserved in pathology collections, remains a largely untapped resource. Here we demonstrate that proteomic profiles are well preserved in FFPE human heart specimens and compatible with high-resolution, quantitative analysis. Quantifying approximately 4,000 proteins per sample, we show this approach effectively distinguishes disease states and subanatomical regions, revealing distinct underlying protein signatures. Specifically, the human sinoatrial node exhibited enrichment of collagen VI and G protein-coupled receptor signaling. Myocardial biopsies from patients with arrhythmogenic cardiomyopathy were characterized by fibrosis and metabolic/cytoskeletal derangements, clearly separating them from donor heart biopsies. This study establishes FFPE heart tissue as a robust resource for cardiac proteomics, enabling retrospective molecular profiling at scale and unlocking archived specimens for disease discovery and precision cardiology.

Indexed as

FixativesFormaldehydeMyocardiumParaffin EmbeddingProteomeProteomicsTissue FixationBiopsyFibrosisHumansMaleFixativesFormaldehydeProteome

Identifiers

PMID41006917
PMCPMC12520988

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.