Evidence map›Paper›PMID 41007934›Full record

ArticleAnimals : an open access journal from MDPI2025

Tissue-Specific Mitochondrial Functionality and Mitochondrial-Related Gene Profiles in Response to Maternal Nutrition and One-Carbon Metabolite Supplementation During Early Pregnancy in Heifers.

Kazi Sarjana Safain, Matthew S Crouse, Mara R Hirchert, Yssi L Entzie, Jessica G Syring, Mojtaba Daneshi, Muhammad Anas, Layla E King, Lawrence P Reynolds, Pawel P Borowicz and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Kazi Sarjana SafainDepartment of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA.ORCID 0000-0001-8742-4890
Matthew S CrouseUnited States Department of Agriculture, Agriculture Research Service, U.S. Meat Animal Research Center, Clay Center, NE 68933, USA.ORCID 0000-0003-4579-2737
Mara R HirchertDepartment of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA.
Yssi L EntzieDepartment of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA.
Jessica G SyringDepartment of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA.ORCID 0009-0004-5134-3422
Mojtaba DaneshiDepartment of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA.ORCID 0000-0003-4980-7502
Muhammad AnasDepartment of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA.ORCID 0009-0005-3791-7335
Layla E KingDepartment of Agriculture and Natural Resources, University of Minnesota Crookston, Crookston, MN 56716, USA.
Lawrence P ReynoldsDepartment of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA.ORCID 0000-0002-6838-7809
Pawel P BorowiczDepartment of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA.ORCID 0000-0001-5436-393X
Carl R DahlenDepartment of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA.ORCID 0000-0002-4556-4315
Alison K WardDepartment of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK S7N 5A2, Canada.ORCID 0000-0002-9774-8677
Joel S CatonDepartment of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA.
Kendall C SwansonDepartment of Animal Sciences, Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58105, USA.ORCID 0000-0002-5694-4778

Funding

USDA-NIFA-AFRI 2018-07055
6 · The paper itself

Abstract

backgroundMitochondria are essential for fetal development, regulating energy metabolism and metabolic programming. This study examined how maternal nutrition and one-carbon metabolite (OCM) supplementation during early gestation affect mitochondrial function in fetal liver and muscle at day 161 of gestation in beef heifers.

methodsTwenty-nine crossbred Angus heifers were assigned to one of four treatments in a 2 × 2 factorial design: control (CON; 0.45 kg/day ADG) or restricted gain (RES; -0.23 kg/day), with or without OCM supplementation. Treatments were applied from breeding to day 63 of gestation, after which all heifers received a common diet. Fetal liver and muscle tissues were collected at day 161. Mitochondrial respiration (Seahorse assay), mtDNA copy number (qPCR), and mitochondria-related gene expression (RNA-seq) were assessed.

resultsIn fetal liver, state 3 respiration was highest in CON + OCM, while state 4o respiration was lowest in RES + OCM (

conclusionsOCM supplementation enhanced mitochondrial respiration and biogenesis in fetal liver, likely via post-translational mechanisms. In contrast, fetal muscle showed downregulation of mitochondria-related genes without functional changes, indicating transcriptional reprogramming with potential effects on later metabolic function. These results underscore early gestation as a critical window for OCM-based nutritional interventions to improve metabolic outcomes in livestock.

Indexed as

beef heifersfetal developmentmaternal nutritionmetabolismmitochondriaone-carbon metabolismtranscriptome

Identifiers

PMID41007934
PMCPMC12466886

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.