Evidence map›Paper›PMID 41007947›Full record

ArticleAnimals : an open access journal from MDPI2025

Development of a 5K Liquid-Phase Genome-Wide Breeding Chip for Xinglong Buffalo.

Yuqing Jiao, Junming Jiang, Shiyuan Li, Taoyu Chen, Xinjun Qiu, Ke Cui, Boling Li, Si Chen, Qiaoling Chen, Li Du and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yuqing JiaoHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.
Junming JiangHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.
Shiyuan LiHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.
Taoyu ChenHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.
Xinjun QiuHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.
Ke CuiThe Hainan Animal Husbandry Technology Promotion Station, Haikou 571100, China.
Boling LiThe Hainan Animal Husbandry Technology Promotion Station, Haikou 571100, China.
Si ChenHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.ORCID 0000-0002-0435-1954
Qiaoling ChenHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.
Li DuHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.
Churiga ManHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.
Lianbin LiHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.
Fengyang WangHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.
Hongyan GaoHainan Key Laboratory for Tropical Animal Breeding and Disease Research, Hainan University, Haikou 570228, China.

Funding

fengyang wang [2025]100
6 · The paper itself

Abstract

The Xinglong buffalo is a local swamp buffalo breed adapted to tropical regions in China. To facilitate the protection and utilization of valuable genetic resources, we first developed the breed-specific single nucleotide polymorphism (SNP) liquid-phase chip based on genotyping-by-target-sequencing (GBTS) technology. Whole-genome resequencing data from 143 buffaloes, resulting in 34,757,694 SNPs, were used to identify 1208 breed-specific and 2889 background sites. This chip also incorporates 965 functional SNP sites derived from literature, including SNPs significantly associated with immunity, reproduction, growth, and production. A total of 5062 SNP sites were successfully identified for the development of a 5K liquid-phase genome-wide breeding chip for the Xinglong buffalo. The validation of the chip using 93 samples showed a high detection rate with good repeatability and consistency. In addition, the chip exhibits strong capabilities in clustering and kinship analysis. Results of kinship analysis underscored the importance of a breed-specific chip for the Xinglong buffalo. These results highlight the advantages of a low-density, cost-effective, and breed-specific SNP chip for accurate genotyping. This chip will support future endeavors in molecular breeding, conservation, and genetic evaluation of Xinglong buffalo, thereby facilitating the sustainable utilization of this valuable indigenous germplasm resource.

Indexed as

genetic evaluationgenotyping by target sequencingsingle nucleotide polymorphism chipXinglong buffalo

Identifiers

PMID41007947
PMCPMC12466515

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.