Evidence mapPaperPMID 41009447Full record

ArticleInternational journal of molecular sciences2025

MicroRNAs Secreted by the Embryo in Spent Culture Medium Can Regulate mRNAs Involved in Endometrial Receptivity, Embryo Attachment, and Invasion.

Angela Caponnetto, Carmen Ferrara, Anna Fazzio, Luca Carli, Cristina Barbagallo, Michele Stella, Davide Barbagallo, Marco Ragusa, Michael Feichtinger, Cinzia Di Pietro and 1 more

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Angela CaponnettoDepartment of Biomedical and Biotechnological Sciences, Section of Biology and Genetics "G. Sichel", University of Catania, 95123 Catania, Italy.ORCID 0009-0009-0959-3133
Carmen FerraraDepartment of Biomedical and Biotechnological Sciences, Section of Biology and Genetics "G. Sichel", University of Catania, 95123 Catania, Italy.ORCID 0000-0003-4915-0854
Anna FazzioDepartment of Biomedical and Biotechnological Sciences, Section of Biology and Genetics "G. Sichel", University of Catania, 95123 Catania, Italy.ORCID 0009-0006-2045-8344
Luca CarliWunschbaby Institut Feichtinger, 1130 Vienna, Austria.ORCID 0009-0000-1992-5220
Cristina BarbagalloDepartment of Biomedical and Biotechnological Sciences, Section of Biology and Genetics "G. Sichel", University of Catania, 95123 Catania, Italy.ORCID 0000-0002-6769-4516
Michele StellaDepartment of Biomedical and Biotechnological Sciences, Section of Biology and Genetics "G. Sichel", University of Catania, 95123 Catania, Italy.
Davide BarbagalloDepartment of Biomedical and Biotechnological Sciences, Section of Biology and Genetics "G. Sichel", University of Catania, 95123 Catania, Italy.ORCID 0000-0001-5331-4554
Marco RagusaDepartment of Biomedical and Biotechnological Sciences, Section of Biology and Genetics "G. Sichel", University of Catania, 95123 Catania, Italy.ORCID 0000-0002-4282-920X
Michael FeichtingerWunschbaby Institut Feichtinger, 1130 Vienna, Austria.ORCID 0000-0001-6453-9281
Cinzia Di PietroDepartment of Biomedical and Biotechnological Sciences, Section of Biology and Genetics "G. Sichel", University of Catania, 95123 Catania, Italy.ORCID 0000-0002-6036-4469
Rosalia BattagliaDepartment of Medicine and Surgery, University of Enna "Kore", 94100 Enna, Italy.

Funding

Merck KGaA Grant for Fertility Innovation 2018
6 · The paper itself

Abstract

MicroRNAs, secreted by the embryo in blastocoel fluid (BF) and embryo spent culture medium (SCM), regulate important cellular pathways controlling the stemness of inner cell mass, trophectoderm differentiation, and the dialogue between blastocyst and maternal tissues. In recent years, their role as non-invasive biomarkers of embryo quality has been deeply investigated. We compared the expression profiles of 96 microRNAs between BF and SCM from the same embryos, highlighting the differences between these two compartments. We found 10 and 6 microRNAs specifically expressed in BF and in SCM, respectively; 22 microRNAs significantly up-regulated in BF; and 2 significantly up-regulated in SCM. To investigate the role of SCM microRNAs in implantation, we focused on the microRNAs specifically expressed/up-regulated in SCM and absent in blank medium. We deepened our understanding of SCM microRNA's biological role by building a network of miRNA-mRNA interaction within the signalling pathways crucial in embryo implantation success. We demonstrated that BF and SCM contain different sets of microRNAs playing different and unique roles in embryo implantation and development. Finally, we suggest that there is not a single "ideal" technique to identify the most competent embryo, but an integrated approach is needed to obtain informative results on the health of the embryo.

Indexed as

Embryo ImplantationEmbryo, MammalianEndometriumMicroRNAsRNA, MessengerAnimalsBlastocystCulture MediaFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalHumansMiceCulture MediaMicroRNAsRNA, Messengercross-talk embryo maternal tissuesembryo culture mediumendometriumimplantationmiRNA

Identifiers

PMID41009447
PMCPMC12469383

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.