The Achilles Heel of Protein Biochemistry: Insolubility of Recombinant Proteins-A Case Study About Producing a Rice Enzyme.

Tibo De Coninck, Hannes Vanhaeren, Els J M Van Damme

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Tibo De ConinckLaboratory for Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Proeftuinstraat 86, 9000 Ghent, Belgium.ORCID 0000-0003-1600-2679

Hannes VanhaerenLaboratory for Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Proeftuinstraat 86, 9000 Ghent, Belgium.

Els J M Van DammeLaboratory for Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, Proeftuinstraat 86, 9000 Ghent, Belgium.ORCID 0000-0001-9848-766X

Funding

Fonds voor Wetenschappelijk Onderzoek Vlaanderen G008619N

6 · The paper itself

Abstract

Biochemical characterization of proteins is fundamental to understanding their function. Typically, research in protein structure/function requires reasonable quantities of the protein of interest. Because of the low abundances in their natural environment, the heterogenous state of post-translational modifications, and the difficulty of obtaining the tissue containing the protein of interest, recombinant protein production is usually employed. One of the major difficulties impeding advances in biotechnological research is protein insolubility, undermining further downstream research and applications.

Indexed as

OryzaPlant ProteinsRecombinant ProteinsEscherichia coliProtein Processing, Post-TranslationalSolubilityPlant ProteinsRecombinant ProteinsA. thalianaE. coliOsAPSEP. pastorisprotein solubilityrecombinant proteins

Identifiers

PMID41009543

DOI10.3390/ijms26188974

PMCPMC12470104

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.