Evidence map›Paper›PMID 41014416›Full record

ArticleFood and environmental virology2025

Discovery of Proteinaceous Attachment Factors for GII.4 Norovirus from the Diarrheal Sample of a Norovirus-Infected Patient.

Jialun Xu, Xiangjun Zhan, Fengqing Wang, Chenang Lyu, Ran An, Joanne Hewitt, Peng Tian, Dapeng Wang

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Article in Food and environmental virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Jialun XuDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, 200240, China.
Xiangjun ZhanDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, 200240, China.
Fengqing WangDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, 200240, China.
Chenang LyuDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, 200240, China.
Ran AnDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, 200240, China.
Joanne HewittInstitute of Environmental Science and Research (ESR Ltd), Porirua, Wellington, New Zealand.
Peng TianAgricultural Research Service-United States Department of Agriculture Produce, Safety and Microbiology Research Unit, Western Regional Research Center, Albany, CA, 94706, USA.
Dapeng WangDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai, 200240, China. dapengwang@sjtu.edu.cn.

Funding

National Key Research and Development Program of China 2024YFD2100900
6 · The paper itself

Abstract

Human norovirus (HuNoV) is the leading cause of acute nonbacterial gastroenteritis globally. Histo-blood group antigens (HBGAs) have been recognized as attachment factors for HuNoV. However, the receptors or other potential attachment factors for HuNoV have not been fully elucidated. A bacterial cell surface-displayed system was used to express GII.4 HuNoV protruding domain (GII.4 P) to capture attachment factors from a diarrheal sample of a patient infected with GII.4 HuNoV. A library of candidate HuNoV proteinaceous attachment factors was constructed. Through a database comparison, a literature review, the predicted protein subcellular localization, the protein-protein interactions (PPIs) network analysis, the molecular simulation and the protein docking analysis, four candidate attachment factors were selected for further investigation from the initial 118 candidates. Finally, two proteins, namely myelin-oligodendrocyte glycoprotein (MOG) and leucine-rich repeat-containing protein 15 (LRRC15), were demonstrated strongly binding to GII.4 P by both in vivo and in vitro assays. The binding between MOG/LRRC15 and GII.4 P can be blocked by GII.4 HuNoV antibody. Immunofluorescence showed that MOG/LRRC15 and GII.4 HuNoV co-localized in the cytoplasm in transfected cells. However, GII.4 HuNoV did not proliferate in the newly constructed MOG/LRRC15 overexpressing cells. This study describes attempts to identify HuNoV proteinaceous attachment factors from the diarrheal sample. Findings from this study will aid in understanding of HuNoV infection in humans, enrichment of GII.4 HuNoV from various environments and construction of cell lines for HuNoV cultivation in vitro.

Indexed as

Caliciviridae InfectionsDiarrheaGastroenteritisNorovirusViral ProteinsHumansProtein BindingVirus AttachmentViral ProteinsBacterial cell surface-displayed systemHuman norovirusLeucine-rich repeat-containing protein 15Myelin-oligodendrocyte glycoproteinProteinaceous attachment factors

Identifiers

PMID41014416

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.