Evidence mapPaperPMID 41014556Full record

ArticleCell reports2025

ER stress tolerance is regulated by copper-dependent PERK kinase activity.

Sarah E Bond Newton, Xinglong Shi, Noah R Beratan, Julia Perhacs, Jitendra K Arya, Margaret K Bond, Tali Gidalevitz, Cagla Akay-Espinoza, Donita C Brady, Kelly L Jordan-Sciutto

Abstract read
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sarah E Bond NewtonWeinberg ALS Center, Farber Institute for Neuroscience, Department of Neuroscience, Thomas Jefferson University, Philadelphia, PA 19107, USA; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Xinglong ShiDepartment of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Noah R BeratanDepartment of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Julia PerhacsDepartment of Biology, College of Arts and Sciences, Drexel University, Philadelphia, PA 19104, USA.
Jitendra K AryaDepartment of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Margaret K BondDepartment of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Tali GidalevitzDepartment of Biology, College of Arts and Sciences, Drexel University, Philadelphia, PA 19104, USA.
Cagla Akay-EspinozaDepartment of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Donita C BradyDepartment of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: bradyd@pennmedicine.upenn.edu.
Kelly L Jordan-SciuttoDepartment of Oral Medicine, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: jordank@upenn.edu.

Funding

Penn Mental Health AIDS Research CenterP30MH097488 · NIMH · UNIVERSITY OF PENNSYLVANIA · 2022 to 2025
$5.0M
STRUCTURAL BIOLOGY TRAINING PROGRAMT32GM008275 · UNIVERSITY OF PENNSYLVANIA · 1988 to 2005
$1.7M
Translational Research Support CoreP30ES013508 · UNIVERSITY OF PENNSYLVANIA · 2025 to 2025
$1.6M
Cell-specific roles for PERK in HIV-induced neuroinflammationR01MH136841 · UNIVERSITY OF PENNSYLVANIA · 2025 to 2025
$746k
Molecular and Cellular Mechanisms of Copper-Dependent Nutrient Signaling and MetabolismR35GM124749 · UNIVERSITY OF PENNSYLVANIA · 2025 to 2025
$488k
ER stress sensor PERK functions in localization of neuronal growth factors through CaMKIIF31NS135921 · DREXEL UNIVERSITY · 2025 to 2025
$50k
NIEHS NIH HHS P30 ES013508NIGMS NIH HHS R35 GM124749NIGMS NIH HHS T32 GM008275NIMH NIH HHS P30 MH097488NIMH NIH HHS R01 MH109382NIMH NIH HHS R01 MH136841NINDS NIH HHS F31 NS135921
6 · The paper itself

Abstract

Pancreatic/PKR-like endoplasmic reticulum (ER) kinase (PERK) is a kinase that, in response to ER stress, mediates dual homeostatic and pro-apoptotic signaling. Thus, intricate regulation is required for physiological function. Attempts to modulate PERK activity have shown that the determinants of adaptive vs. maladaptive signaling remain ambiguous. Here, with purified protein, we provide evidence that PERK binds copper, identifies residues required for interaction, and demonstrates that copper is necessary for kinase activity. Furthermore, cellular PERK activity can be modulated via copper availability, and this regulatory relationship can be manipulated to dictate ER stress tolerance. Critically, these phenomena translate to phenotypes in vivo, as C. elegans harboring a "PERK-copper mutant" exhibit exacerbated ER-stress sensitivity. The copper-PERK paradigm suggests that copper homeostasis, as a regulator of PERK, may constitute a critical factor in resolving the long-standing ambiguity in endeavors to therapeutically target PERK.

Indexed as

Caenorhabditis elegans ProteinsCoppereIF-2 KinaseEndoplasmic Reticulum StressAnimalsCaenorhabditis elegansEndoplasmic ReticulumHumansSignal TransductionCaenorhabditis elegans ProteinsCoppereIF-2 KinasecopperCP: Cell biologyER stressISRkinase regulationPERKstress toleranceUPR

Identifiers

PMID41014556
PMCPMC12599989

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.