Evidence mapPaperPMID 41017429Full record

ArticleACS biomaterials science & engineering2025

Small Extracellular Vesicles Released from ARPE-19 Cells Grown under Diabetic Retinopathy Conditions Promote NLRP3 Inflammasome Activation.

Henry H Louie, Odunayo O Mugisho, Lawrence W Chamley, Ilva D Rupenthal

Abstract read
In one paragraph

Article in ACS biomaterials science & engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Henry H LouieBuchanan Ocular Therapeutics Unit, Department of Ophthalmology, Aotearoa-New Zealand National Eye Centre, University of Auckland, Auckland 1142, New Zealand.ORCID 0000-0002-2641-5744
Odunayo O MugishoBuchanan Ocular Therapeutics Unit, Department of Ophthalmology, Aotearoa-New Zealand National Eye Centre, University of Auckland, Auckland 1142, New Zealand.
Lawrence W ChamleyHub for Extracellular Vesicle Investigations, University of Auckland, Auckland 1142, New Zealand.
Ilva D RupenthalBuchanan Ocular Therapeutics Unit, Department of Ophthalmology, Aotearoa-New Zealand National Eye Centre, University of Auckland, Auckland 1142, New Zealand.ORCID 0000-0001-5997-5994

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome is a multiprotein complex that forms part of the innate immune system. Recent studies have shown that the inflammasome pathway is upregulated in diabetic retinopathy (DR), resulting in breakdown of the retinal pigment epithelium (RPE) barrier. It has been hypothesized that small extracellular vesicles (sEVs) may transport inflammasome-related cargo between cells, which in turn contributes to the DR pathogenesis. The aim of this study was to investigate whether sEVs released from ARPE-19 cells grown under basal, DR-like conditions, and DR-like conditions with Peptide 5 treatment differ in inflammasome cargo and uptake. Additionally, the effects of the released sEVs on inflammasome activation in ARPE-19 cells, as well as their effect on vascular growth in a choroidal explant model, were investigated. Results demonstrated that sEVs from ARPE-19 cells grown under DR-like conditions carried increased inflammasome-related cargo and promoted NLRP3 inflammasome activation in recipient cells and tissues. Conversely, sEVs released from cells grown under basal and DR-like conditions with Peptide 5 treatment carried less inflammasome-related cargo. This research provides insights into the role of sEVs in the DR pathogenesis and suggests the potential therapeutic use of certain sEVs in treating DR.

Indexed as

Diabetic RetinopathyExtracellular VesiclesInflammasomesNLR Family, Pyrin Domain-Containing 3 ProteinRetinal Pigment EpitheliumCell LineHumansInflammasomesNLR Family, Pyrin Domain-Containing 3 ProteinNLRP3 protein, humandiabetic retinopathyNLRP3 inflammasomeretinal pigment epitheliumsmall extracellular vesicles

Identifiers

PMID41017429
PMCPMC12532093

What Socratic holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.