ArticleFrontiers in plant science2025
Integrated metabolomic and transcriptomic analysis elucidates transcriptional regulation of flavonoid biosynthesis in differentially pigmented honeysuckle (
Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Ethylene-driven enhancement of bioactive metabolites and in vitro functionality in soybean (Glycine max (L.) Merr.) and mung bean (Vigna radiata (L.) Wilczek) leaves grown in vertical farms: a comparative study.BMC plant biology · 2026Article
- Heterologous expression of the honeysuckle R2R3-MYB transcription factor LjMYB106 enhances phenylpropanoid and flavonoid metabolism inFrontiers in plant science · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Honeysuckle ( Methods: A comparative analysis was conducted using two distinct honeysuckle varieties-'Luyu No.1' (white-yellow flowers) and 'Honghua' (red-pink flowers)-at the optimal harvest stage ('Dabai period'). An integrated transcriptomics (RNA-seq) and widely targeted metabolomics approach was employed to identify differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) between the varieties. Results: Transcriptomic profiling revealed 5,901 DEGs, with significant enrichment in the phenylpropanoid and flavonoid biosynthesis pathways. Key structural genes (PAL, CHS, F3H, FLS, DFR, and ANS) were markedly up-regulated in 'Honghua'. Metabolomic analysis identified 399 flavonoids, including 228 DAMs. The 'Honghua' variety exhibited a 2.32-fold higher total flavonoid content, with substantially elevated levels of anthocyanidins (87.58-fold), flavanols (7.66-fold), and chalcones (6.17-fold). Critical medicinal compounds such as quercetin-3-O-rhamnoside and luteolin-7-O-glucoside were significantly enriched in 'Honghua'. Integrated analysis highlighted the co-expression of transcription factors (MYB, bHLH, NAC) with flavonoid pathways. Crucially, the anthocyanin-modifying enzymes F3'M and 3RT were up-regulated by 22.12- and 3.03-fold, respectively, in 'Honghua', while a potential repressor, TCP15 (bHLH), was down-regulated. Discussion: The results demonstrate that the red-pink pigmentation and superior medicinal quality of 'Honghua' are driven by a coordinated gene-metabolite network that fluxes metabolic intermediates towards anthocyanin and other flavonoid end-products. The pronounced up-regulation of F3'M and 3RT is identified as a key biochemical determinant for anthocyanin diversification and color development. This study provides the first comprehensive framework for flavonoid biosynthesis in honeysuckle, establishing a genetic basis for breeding cultivars with high flavonoid content and enhanced pharmacological value.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.