Evidence map›Paper›PMID 41021139›Full record

ArticleNeurochemical research2025

Hesperidin Attenuates Chronic Stress-Induced Depression via 5-HT2A-Linked Modulation of Neurochemical, Oxidative, and Inflammatory Pathways: Experimental and In Silico Evidence.

Mimansa Kandhwal, Amarjot Kaur Grewal, Varinder Singh, Ojashvi Sharma, Heena Khan, Manjinder Singh, Amit Kumar, Thakur Gurjeet Singh, Tanveer Singh, Sheikh F Ahmad and 2 more

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Article in Neurochemical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Mimansa KandhwalChitkara College of Pharmacy, Chitkara University, Punjab, India.
Amarjot Kaur GrewalChitkara College of Pharmacy, Chitkara University, Punjab, India. amarjot.kaur@chitkara.edu.in.
Varinder SinghDepartment of Pharmaceutical Sciences and Technology, Maharaja Ranjit Singh Punjab Technical University, Bathinda, Punjab, India. varinderjassal17@gmail.com.
Ojashvi SharmaChitkara College of Pharmacy, Chitkara University, Punjab, India.
Heena KhanChitkara College of Pharmacy, Chitkara University, Punjab, India.
Manjinder SinghChitkara College of Pharmacy, Chitkara University, Punjab, India.
Amit KumarChitkara College of Pharmacy, Chitkara University, Punjab, India.
Thakur Gurjeet SinghChitkara College of Pharmacy, Chitkara University, Punjab, India.
Tanveer SinghDepartment of Neuroscience and Experimental Therapeutics, College of Medicine, Texas A&M Health Science Center, College Station, TX, 77807, USA.
Sheikh F AhmadDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, 11451, Riyadh, Saudi Arabia.
Haneen A Al-MazrouaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, 11451, Riyadh, Saudi Arabia.
Gamaleldin I HarisaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, 11451, Riyadh, Saudi Arabia.

Funding

King Saud University ORF-2025-1037
6 · The paper itself

Abstract

Depression is associated with monoaminergic dysregulation, oxidative stress, and neuroinflammation, with the 5-hydroxytryptamine 2 A (5-HT2A) receptor playing a key role. The present study investigated the antidepressant-like potential of hesperidin (HSP), a citrus-derived flavonoid, administered chronically (100 or 200 mg/kg, orally once daily for 21 days) in mice exposed to chronic unpredictable mild stress (CUMS). These effects were further explored through 5-HT2A-associated neurochemical and molecular mechanisms, highlighting its role in stress-related neuroprotection. Exposure to CUMS produced depressive-like behavior, accompanied by increased corticosterone, oxidative stress, inflammation, and depletion of 5-HT and dopamine. Treatment with HSP effectively reversed these alterations by restoring sucrose preference, reducing immobility time in the forced swim test, and normalizing locomotor activity in the open field test. At the neurochemical level, HSP treatment reinstated 5-HT and dopamine levels, reduced corticosterone, and attenuated oxidative (MDA, GSH, SOD, catalase) and inflammatory (NF-κB, IL-1β, IL-6, TNF-α) markers. Moreover, HSP improved neuronal architecture, underscoring its neuroprotective potential. Co-administration of the 5-HT2A receptor agonist, (±)-2,5-dimethoxy-4-iodoamphetamine hydrochloride (DOI, 5 mg/kg, subcutaneously, once daily during the 21-day protocol) abolished HSP's effects, implicating 5-HT2A antagonism in its mechanism. In silico studies confirmed strong and stable binding of HSP to 5-HT2A receptors (- 72.99 kcal/mol), with key interactions involving Trp151, Asp155, Ser159, and Phe340. Molecular dynamics simulations (100 ns) supported complex stability. These results suggest that HSP exerts antidepressant-like effects by modulating 5-HT2A receptors, restoring HPA axis balance, reducing oxidative stress and neuroinflammation, and normalizing monoaminergic function.

Indexed as

Antidepressive AgentsDepressionHesperidinOxidative StressReceptor, Serotonin, 5-HT2AStress, PsychologicalAnimalsInflammationMaleMiceMolecular Docking SimulationAntidepressive AgentsHesperidinReceptor, Serotonin, 5-HT2A5HT2A5-HT receptorChronic unpredictable mild stressDepressionHesperidinInflammation

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.