Evidence map›Paper›PMID 41028452›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

A Brief History of the Pichia pastoris Expression System.

James M Cregg, Thomas G Chappel, Kurt R Gehlsen, Shaun A Kirkpatrick, Knut Madden

Abstract readHistorical Article
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

James M CreggBiogrammatics Inc., Carlsbad, CA, USA.
Thomas G ChappelBiogrammatics Inc., Carlsbad, CA, USA.
Kurt R GehlsenGehlsen Consulting, Palm Beach County, FL, USA.
Shaun A KirkpatrickResearch Corporations Technologies, Tucson, AZ, USA.
Knut MaddenBiogrammatics Inc., Carlsbad, CA, USA. knut.madden@biogrammatics.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The origin and development of the Pichia pastoris expression system is described from its roots as an organism for the production of single-celled protein (SCP) to its evolution into a major host for the synthesis of recombinant proteins. Throughout its history, the ability of P. pastoris to grow on methanol as a sole source of carbon and energy to extremely high densities in fermenter cultures has been a central theme. The expression system would not be what it is today without the efforts of numerous talented researchers in fields from molecular biology to bioprocess engineering. As products made using P. pastoris take their places in world markets, it is clear that the future of the system is bright.

Indexed as

PichiaRecombinant ProteinsSaccharomycetalesFermentationGene ExpressionHistory, 20th CenturyHistory, 21st CenturyMethanolMethanolRecombinant ProteinsAlcohol oxidase promoterDNA-mediated transformationForeign gene expressionGlycosylationHigh-density fermentationInvertaseKomagataella phaffiiPichia pastorisRecombinant protein productionβ-galactosidase

Identifiers

PMID41028452

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.