Evidence mapPaperPMID 41034521Full record

ArticleNature cell biology2025

Microtubule architecture connects AMOT stability to YAP/TAZ mechanotransduction and Hippo signalling.

Giada Vanni, Anna Citron, Ambela Suli, Paolo Contessotto, Robin Caire, Alessandro Gandin, Giovanna Mantovan, Francesca Zanconato, Giovanna Brusatin, Michele Di Palma and 7 more

Abstract read
In one paragraph

Article in Nature cell biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
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  6. Article
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  9. Review
  10. Review
  11. Stress transmission towards the nucleus of the cell.Frontiers in cell and developmental biology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Giada VanniDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Anna CitronDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Ambela SuliDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Paolo ContessottoDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Robin CaireDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Alessandro GandinDepartment of Industrial Engineering, University of Padova, Padova, Italy.
Giovanna MantovanDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Francesca ZanconatoDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Giovanna BrusatinDepartment of Industrial Engineering, University of Padova, Padova, Italy.ORCID http://orcid.org/0000-0002-5219-8376
Michele Di PalmaDepartment of Biomedical Sciences, University of Padova, Padova, Italy.
Elisa PeiranoDepartment of Biomedical Sciences, University of Padova, Padova, Italy.
Lisa Sofia PozzerDepartment of Biomedical Sciences, University of Padova, Padova, Italy.
Carlo AlbaneseDepartment of Molecular Medicine, University of Padova, Padova, Italy.
Roberto A SteinerDepartment of Biomedical Sciences, University of Padova, Padova, Italy.
Michelangelo CordenonsiDepartment of Molecular Medicine, University of Padova, Padova, Italy.ORCID http://orcid.org/0000-0002-8253-5724
Tito PancieraDepartment of Molecular Medicine, University of Padova, Padova, Italy. tito.panciera@unipd.it.ORCID http://orcid.org/0000-0002-9272-5716
Stefano PiccoloDepartment of Molecular Medicine, University of Padova, Padova, Italy. stefano.piccolo@unipd.it.ORCID http://orcid.org/0000-0002-1600-3092

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cellular mechanotransduction is a key informational system, yet its mechanisms remain elusive. Here we unveil the role of microtubules in mechanosignalling, operating downstream of subnuclear F-actin and nuclear envelope mechanics. Upon mechanical activation, microtubules reorganize from a perinuclear cage into a radial array nucleated by centrosomes. This structural rearrangement triggers degradation of AMOT proteins, which we identify as key mechanical rheostats that sequester YAP/TAZ in the cytoplasm. AMOT is stable in mechano-OFF but degraded in mechano-ON cell states, where microtubules allow AMOT rapid transport to the pericentrosomal proteasome in complex with dynein/dynactin. This process ensures swift control of YAP/TAZ function in response to changes in cell mechanics, with experimental loss of AMOT proteins rendering cells insensitive to mechanical modulations. Ras/RTK oncogenes promote YAP/TAZ-dependent tumorigenesis by corrupting this AMOT-centred mechanical checkpoint. Notably, the Hippo pathway fine-tunes mechanotransduction: LATS kinases phosphorylate AMOT, shielding it from degradation, thereby indirectly restraining YAP/TAZ. Thus, AMOT protein stability serves as a hub linking cytoskeletal reorganization and Hippo signalling to YAP/TAZ mechanosignalling.

Indexed as

Adaptor Proteins, Signal TransducingIntracellular Signaling Peptides and ProteinsMechanotransduction, CellularMicrotubulesProtein Serine-Threonine KinasesTranscription FactorsAcyltransferasesAnimalsCell Cycle ProteinsHippo Signaling PathwayHumansMicePhosphorylationProtein StabilityProteolysisSignal TransductionAcyltransferasesAdaptor Proteins, Signal TransducingCell Cycle ProteinsIntracellular Signaling Peptides and ProteinsProtein Serine-Threonine KinasesTrans-ActivatorsTranscriptional Coactivator with PDZ-Binding Motif ProteinsTranscription FactorsWWTR1 protein, humanYAP1 protein, humanYAP-Signaling Proteins

Identifiers

PMID41034521
PMCPMC12527920

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.