ArticleOpen veterinary journal2025
Harnessing bioinformatics and recombinant technology to combat avian coccidiosis: A 19-kDa sporozoite protein-based vaccine.
Article in Open veterinary journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Anticoccidial Efficacy of Solid-Dispersion Formulations ContainingVeterinary sciences · 2026Article
- A Tetravalent Recombinant Subunit Vaccine Provides Protection Against Mixed Challenges with FourAnimals : an open access journal from MDPI · 2026Article
- Microscopic and molecular identification ofOpen veterinary journal · 2026Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Coccidiosis is an economically important disease caused by Aim: This study aimed to design, express, and evaluate a recombinant 19-kDa sporozoite protein (E19kDa) as a vaccine candidate against mixed Methods: The study used bioinformatic analysis, protein expression analysis, immunization trials, and immune response evaluation were performed. Protein properties were predicted using bioinformatics tools. Gene 838 (Accession No. PV347147) was cloned into a pET-28b plasmid and expressed in Results: The results showed high expression of E19kDa at 1 mM IPTG. SDS-PAGE confirmed the purified protein at ~29kDa. Immunized birds exhibited better weight gain, lower oocyst counts (10,245 versus 22,755), and increased white blood cell counts and IgY titers compared with infected controls. Enzyme-linked immunosorbent assay confirmed a peak antibody response on day 28 post-vaccination. The vaccine significantly reduced clinical symptoms and mortality. Conclusion: This study highlights the potential of the recombinant E19kDa protein as a promising candidate vaccine against coccidiosis. The approach combining bioinformatics, molecular expression, and immunological testing offers a cost-effective solution for the disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.