Evidence map›Paper›PMID 41036852›Full record

ArticleJournal of virology2025

Tankyrases positively regulate influenza A virus replication via type I interferon response.

Gayan Bamunuarachchi, Kishore Vaddadi, Yurong Liang, Zhengyu Zhu, Quanjin Dang, Sankha Hewawasam, Lin Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. NADThe Journal of general virology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Gayan Bamunuarachchi *Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA.
Kishore Vaddadi *Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA.ORCID 0000-0003-0320-9064
Yurong LiangOklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA.
Zhengyu ZhuOklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA.
Quanjin DangOklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA.
Sankha HewawasamOklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA.
Lin LiuOklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA.ORCID 0000-0002-4811-4897

Funding

ZFC3H1 Regulation of Host Defense and Influenza A Virus PathogenesisP20GM103648 · NIGMS · OKLAHOMA STATE UNIVERSITY STILLWATER · PI CHANNAPPANAVAR, RUDRAGOUDA · 2013 to 2022
$22.3M
Pilot Project Grant ProgramP30GM149368 · NIGMS · OKLAHOMA STATE UNIVERSITY STILLWATER · PI LIN LIU · 2023 to 2026
$5.5M
The Role of Iron In Pulmonary FibrosisR01HL157450 · NHLBI · OKLAHOMA STATE UNIVERSITY STILLWATER · PI LIN LIU · 2023 to 2026
$2.0M
The Role of LncRNAs in Pulmonary FibrosisR01HL135152 · NHLBI · OKLAHOMA STATE UNIVERSITY STILLWATER · PI LIU, LIN · 2017 to 2020
$1.5M
Lnc-PINK regulation of innate immunity in lung epithelial cellsR21AI152004 · NIAID · OKLAHOMA STATE UNIVERSITY STILLWATER · PI LIU, LIN · 2021 to 2022
$411k
MicroRNA-193b regulation of influenza virus replicationR21AI121591 · NIAID · OKLAHOMA STATE UNIVERSITY STILLWATER · PI LIU, LIN · 2016 to 2017
$401k
NHLBI NIH HHS R01 HL135152NHLBI NIH HHS R01 HL157450NIAID NIH HHS R21 AI121591NIAID NIH HHS R21 AI152004NIGMS NIH HHS P20 GM103648NIGMS NIH HHS P30 GM149368
6 · The paper itself

Abstract

Influenza viruses are respiratory pathogens that cause both seasonal and unpredictable pandemic infections in humans. Currently approved anti-influenza drugs target the viral proteins. A recurrent mutation in the influenza genome leads to drug resistance and thus hinders the efficacy of anti-influenza drug treatments. Influenza A virus (IAV) depends on host factors to complete its virus life cycle. Thus, there are increasing interests in antiviral drugs that target host cellular proteins required for virus replication. Poly (ADP-ribose) polymerases (PARPs) are the host factors that modify protein functions by adding ADP-ribose to target proteins. Using the CRISPR activation system, we screened all 17 PARP members for its effects on IAV infection in lung epithelial A549 cells. Tankyrase 1 and 2 (TNKS 1/2 or PARP5A/5B) were found to be potent proviral factors. Knockout of TNKS1 or TNKS2 in HEK293T cells by CRISPR reduced viral mRNA and protein levels in cells and viral titers in culture media. Double knockout of TNKS1 and TNKS2 had a larger effect on IAV infection than single knockout of each isoform. The effect of TNKS double knockout on IAV replication was strain independent. Overexpression of TNKS1/2 in the double knockout cells restored the IAV replication to a level similar to the control cells. TNKS double knockout activated JNK/c-Jun signaling, enhanced Stat signaling, and increased type I interferon expression. Finally,

Indexed as

Influenza A virusInterferon Type ITankyrasesVirus ReplicationA549 CellsAnimalsHEK293 CellsHost-Pathogen InteractionsHumansInfluenza, HumanMiceMice, Inbred C57BLOrthomyxoviridae InfectionsPoly(ADP-ribose) PolymerasesInterferon Type IPoly(ADP-ribose) PolymerasesTankyrasesinfluenza A virusPoly (ADP-ribose) polymerasespost-translational modificationsTNKS1TNKS2type I interferon response

Identifiers

PMID41036852
PMCPMC12548432

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.