Evidence map›Paper›PMID 41037091›Full record

ArticleOdontology2026

Experimental in vitro simulation of the impact of e-cigarette vapors on enamel and dentin.

Davi Ariel Nobuo Bepu, Alice Corrêa Silva-Sousa, Fabiane Carneiro Lopes-Olhê, Débora Fernandes Costa Guedes, André Luís Faria-E-Silva, Jardel Francisco Mazzi-Chaves, Manoel Damião Sousa-Neto, Aline Evangelista Souza-Gabriel

Abstract read
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In one paragraph

Article in Odontology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Davi Ariel Nobuo BepuDepartment of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Av. Do Café, S/N., Ribeirão Preto, São Paulo, 14020-904, Brazil.ORCID http://orcid.org/0000-0002-8262-0515
Alice Corrêa Silva-SousaDepartment of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Av. Do Café, S/N., Ribeirão Preto, São Paulo, 14020-904, Brazil.ORCID http://orcid.org/0000-0002-9931-0935
Fabiane Carneiro Lopes-OlhêDepartment of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Av. Do Café, S/N., Ribeirão Preto, São Paulo, 14020-904, Brazil.ORCID http://orcid.org/0000-0003-4375-3371
Débora Fernandes Costa GuedesDepartment of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Av. Do Café, S/N., Ribeirão Preto, São Paulo, 14020-904, Brazil.ORCID http://orcid.org/0000-0002-2221-6860
André Luís Faria-E-SilvaFaculty of Dentistry, Université Laval, Québec City, QC, Canada.ORCID http://orcid.org/0000-0003-3846-4786
Jardel Francisco Mazzi-ChavesDepartment of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Av. Do Café, S/N., Ribeirão Preto, São Paulo, 14020-904, Brazil.ORCID http://orcid.org/0000-0002-9889-1790
Manoel Damião Sousa-NetoDepartment of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Av. Do Café, S/N., Ribeirão Preto, São Paulo, 14020-904, Brazil. sousanet@forp.usp.br.ORCID http://orcid.org/0000-0002-7696-7600
Aline Evangelista Souza-GabrielDepartment of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo (USP), Av. Do Café, S/N., Ribeirão Preto, São Paulo, 14020-904, Brazil.ORCID http://orcid.org/0000-0002-9280-2945

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 33002029032P4
6 · The paper itself

Abstract

The aim of this study was to evaluate changes in tooth color and the bond strength of resin to enamel and dentin after exposure to electronic cigarette (e-cigarette) vapor in an in vitro vaping model, as well as to analyze the chemical composition of the materials present in the vapor. A device with a vacuum pump simulated vaping. Eighty dental slabs (40 dentin and 40 enamel) and were randomly divided into two groups. Half received e-cigarette exposure, and the other remained without vaping (control). Color changes were measured using a spectrophotometer (CIELAB). Composite cylinders were built on substrates using etch-and-rinse or self-etch strategies and subjected to loading tests. Gas chromatography-mass spectrometry (GC-MS) analyzed the organic compound of the e-liquid, while inductively coupled plasma mass spectrometry (ICP-MS) assessed the metal content. Statistical analysis was conducted using MANOVA and ANOVA (α = 0.05), and CIEDE2000 formula. E-cigarette exposure darkened (L*) and yellowed (b*) enamel and dentin. Bond strength in dentin decreased for both adhesion strategies, and in enamel using the etch-and-rinse adhesive. GC-MS identified 72 different volatile compounds, whereas ICP-MS detected 26 distinct metals. Among metals, eight exceeded the WHO (World Health Organization) tolerance limits. E-cigarette exposure altered the color of substrates and reduced resin bond strength in dentin for both adhesives, and in enamel restored with the etch-and-rinse technique. E-liquid had toxic organic compounds and metals. Exposure to e-cigarette can cause tooth discoloration and weaken bonding to dental tissues. Toxic volatile organic and metallic compounds present in vapor can adversely affect oral health.

Indexed as

Dental EnamelDentinE-Cigarette VaporElectronic Nicotine Delivery SystemsColorGas Chromatography-Mass SpectrometryHumansIn Vitro TechniquesE-Cigarette VaporBond StrengthDentinElectronic cigaretteEnamelTooth discoloration

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.