Evidence map›Paper›PMID 41044994›Full record

ArticleOrthodontics & craniofacial research2025

Differentially Expressed Salivary miRNAs in Temporomandibular Disorders.

Karolina Elżbieta Kaczor-Urbanowicz, Mohammad Aziz, Holly Wilhalme, Sharon Aronovich, Elizabeth Rae Hatfield, Tara Aghaloo, Sherwin Arman, Aida Mohammadi, Mohammed Husain, Sotirios Tetradis and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Orthodontics & craniofacial research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Karolina Elżbieta Kaczor-UrbanowiczCenter for Oral and Head/Neck Oncology Research, UCLA School of Dentistry, University of California at Los Angeles, Los Angeles, California, USA.ORCID https://orcid.org/0000-0001-9243-3652
Mohammad AzizCenter for Oral and Head/Neck Oncology Research, UCLA School of Dentistry, University of California at Los Angeles, Los Angeles, California, USA.
Holly WilhalmeUCLA Department of Medicine Statistics Core (UCLA DOMStat), University of California at Los Angeles, Los Angeles, California, USA.
Sharon AronovichDepartment of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA.
Elizabeth Rae HatfieldDepartment of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA.
Tara AghalooSection of Oral and Maxillofacial Surgery, Division of Diagnostic and Surgical Sciences, UCLA School of Dentistry, Los Angeles, California, USA.
Sherwin ArmanUCLA Orofacial Pain Clinic, UCLA School of Dentistry, Los Angeles, California, USA.
Aida MohammadiUCLA Section of Orthodontics, UCLA School of Dentistry, University of California at Los Angeles, Los Angeles, California, USA.
Mohammed HusainOral and Maxillofacial Radiology, UCLA School of Dentistry, Los Angeles, California, USA.
Sotirios TetradisOral and Maxillofacial Radiology, UCLA School of Dentistry, Los Angeles, California, USA.
David ElashoffUCLA Department of Medicine Statistics Core (UCLA DOMStat), University of California at Los Angeles, Los Angeles, California, USA.
Matteo PellegriniUCLA Institute for Quantitative and Computational Biosciences, University of California at Los Angeles, California, Los Angeles, USA.ORCID https://orcid.org/0000-0001-9355-9564
David T W WongCenter for Oral and Head/Neck Oncology Research, UCLA School of Dentistry, University of California at Los Angeles, Los Angeles, California, USA.ORCID https://orcid.org/0000-0002-0290-2206
Sunil KapilaUCLA Section of Orthodontics, UCLA School of Dentistry, University of California at Los Angeles, Los Angeles, California, USA.

Funding

Interdisciplinary Clinical Advances and Research Excellence in TMDs (ICARE 4 TMDs) CollaborativeR34DE033595 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KAPILA, SUNIL D · 2023 to 2023
$312k
NIDCR NIH HHS R34 DE033595NIH HHS NIH/NIDCR R25 DE030117-02NIH HHS NIH/NIDCR R34DE033595-01NIH HHS NIH/NIDCR UH2 DE032208-01NIH HHS NIH/NIDCR UH3 DE032208-04NIH HHS QCBio Collaboratory Fellowship 2024/25 to KEK-U
6 · The paper itself

Abstract

objectiveTemporomandibular disorders (TMDs) are heterogeneous conditions of unclear aetiology involving the temporomandibular joint, masticatory muscles and neural tissues. Limited understanding of their pathogenesis hampers accurate diagnosis and targeted treatment. Therefore, this study aimed to identify salivary microRNA (miRNA) signatures associated with TMDs to support future diagnostic, therapeutic and prognostic applications. MATERIALS AND

methodsUnstimulated cell-free saliva (5 mL) was collected from 9 adult female TMD subjects (using Diagnostic Criteria/TMD) and eight healthy female controls of similar ages. Total RNA was extracted, small RNA libraries were prepared, and sequencing was performed using Illumina NovaSeq 6000. Reads were aligned to the human genome (GRCh38) via STAR. Differential expression analysis was conducted using DESeq2, followed by functional enrichment via miEAA 2.1.

resultsA total of 187 salivary miRNAs were significantly differentially expressed between TMD and control groups (adjusted p < 0.05; log2-fold change > +1 or < -1), with 125 upregulated and 62 downregulated in TMD subjects. Several differentially expressed miRNAs were linked to the negative regulation of cadherin-mediated cell-cell adhesion, neurogenesis and chemokine production. Some overlapped with miRNAs implicated in rheumatoid arthritis and osteoarthritis, suggesting shared mechanisms. While no clear association was found between miRNA and TMD phenotypes, 5 miRNAs were strongly (R = 0.67-0.77) and significantly (p < 0.05) correlated with pain intensity and chronic pain grade.

conclusionsSalivary miRNA profiling offers promise as a non-invasive diagnostic tool for TMDs, with the potential to uncover molecular endotypes and disease mechanisms not evident through clinical evaluation. Future studies with larger, more diverse cohorts are needed to validate these findings and assess their clinical utility.

Indexed as

MicroRNAsSalivaTemporomandibular Joint DisordersAdultCase-Control StudiesFemaleGene Expression ProfilingHumansMiddle AgedMicroRNAsbiomarkersmiRNAssalivatemporomandibular disorder (TMD)

Identifiers

PMID41044994
PMCPMC12927133

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.