Evidence map›Paper›PMID 41050708›Full record

ArticleFrontiers in immunology2025

Heterologous prime-boost vaccination with VLA2001 and an ORFV-based vector enhances spike- and nucleocapsid-specific immunity in mice.

Alena Reguzova, Verena Haug, Carina Metz, Melanie Müller, Madeleine Fandrich, Alex Dulovic, Ralf Amann

Abstract read
In one paragraph

Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Alena ReguzovaInstitute of Immunology, University Hospital Tübingen, Tübingen, Germany.
Verena HaugInstitute of Immunology, University Hospital Tübingen, Tübingen, Germany.
Carina MetzInstitute of Immunology, University Hospital Tübingen, Tübingen, Germany.
Melanie MüllerInstitute of Immunology, University Hospital Tübingen, Tübingen, Germany.
Madeleine FandrichNMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany.
Alex DulovicNMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany.
Ralf AmannInstitute of Immunology, University Hospital Tübingen, Tübingen, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Heterologous prime-boost vaccination has emerged as a promising approach to enhance immune responses by combining vaccines with complementary mechanisms of antigen delivery and immune activation. Here, we evaluated the immunogenicity of heterologous regimens combining the licensed inactivated SARS-CoV-2 vaccine (VLA2001) with the replication-deficient Orf virus-based vector vaccine (Prime-2-CoV). Using a mouse model, we compared these regimens to homologous vaccinations with each vaccine alone. Among the combinations tested, priming with VLA2001 followed by boosting with Prime-2-CoV induced the strongest spike-specific antibody responses, superior ACE2-binding inhibition against pre-Omicron variants, and robust Th1-biased immunity, with robust CD4

Indexed as

COVID-19COVID-19 VaccinesNucleocapsidSARS-CoV-2Spike Glycoprotein, CoronavirusAnimalsAntibodies, NeutralizingAntibodies, ViralCD8-Positive T-LymphocytesFemaleGenetic VectorsHumansImmunization, SecondaryMiceMice, Inbred BALB CTh1 CellsAntibodies, NeutralizingAntibodies, ViralCOVID-19 VaccinesSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2Vaccines, InactivatedVLA2001 COVID-19 vaccineheterologous prime-boostOrf virusSARS-CoV-2vaccinesviral vectorVLA2001

Identifiers

PMID41050708
PMCPMC12488559

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.