Evidence map›Paper›PMID 41073005›Full record

ArticleAnalytica chimica acta2025

MALDI matrix sublimation versus spray coating in the FluoMALDI imaging pipeline.

Tae-Hun Hahm, Wanyue Wang, Dalton R Brown, Caitlin M Tressler, Scot C Kuo, Kristine Glunde

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytica chimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tae-Hun HahmApplied Imaging Mass Spectrometry Core, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA; Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Wanyue WangApplied Imaging Mass Spectrometry Core, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA; Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Dalton R BrownApplied Imaging Mass Spectrometry Core, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA; Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Caitlin M TresslerApplied Imaging Mass Spectrometry Core, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA; Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Scot C KuoMicroscope Facility, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA; Sidney Kimmel Comprehensive Cancer Cancer, Johns Hopkins University School of Medicine, Baltimore, MD, 21231, USA.
Kristine GlundeApplied Imaging Mass Spectrometry Core, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA; Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA; Sidney Kimmel Comprehensive Cancer Cancer, Johns Hopkins University School of Medicine, Baltimore, MD, 21231, USA. Electronic address: kglunde1@jhmi.edu.

Funding

Translational Research Central ServicesP30CA006973 · NCI · JOHNS HOPKINS UNIVERSITY · PI ALAN KEITH MEEKER · 1985 to 2026
$208.6M
Imaging Mass Spectrometry-Based Metabolomic Analysis of the Alzheimer's BrainR01AG078830 · NIA · JOHNS HOPKINS UNIVERSITY · PI VALINA L. DAWSON, Kristine Glunde · 2022 to 2026
$4.1M
Reprogramming of creatine metabolism in breast cancer metastasisR01CA264901 · NCI · JOHNS HOPKINS UNIVERSITY · PI Kristine Glunde · 2022 to 2026
$1.8M
timsTOF fleX with MALDI-2 for Advanced Mass Spectrometry ImagingS10OD030500 · OD · JOHNS HOPKINS UNIVERSITY · PI GLUNDE, KRISTINE · 2021 to 2021
$1.3M
LSM880 Laser-scanning Microscope with Airyscan ModuleS10OD023548 · OD · JOHNS HOPKINS UNIVERSITY · PI KUO, SCOT CHARLES · 2018 to 2018
$439k
NCI NIH HHS P30 CA006973NCI NIH HHS R01 CA264901NIA NIH HHS R01 AG078830NIH HHS S10 OD023548NIH HHS S10 OD030500
6 · The paper itself

Abstract

backgroundThis study advances the FluoMALDI pipeline for tissue autofluorescence applications by integrating slide-scanning fluorescence microscopy (SSFM) with matrix-assisted laser desorption/ionization (MALDI) imaging to target autofluorescent tissue structures.

resultsMouse brain, liver, and kidney tissues were used to systematically compare the two matrix deposition methods of sublimation and spray coating in the FluoMALDI pipeline. Half of each tissue section was left uncoated as control by covering it during matrix application. Six MALDI matrices including 9-aminoacridine, norharmane, α-cyano-4-hydroxycinnamic acid, 2,5-dihydroxyacetophenone, 2,5-dihydroxybenzoic acid, or sinapinic acid were applied in equal amounts. Autofluorescence enhancements were evaluated using three fluorescence channels in the green, red, and far-red range of the spectrum, revealing matrix- and tissue-dependent fluorescence enhancement profiles. Norharmane matrix resulted in the most consistent tissue autofluorescence enhancements across deposition methods, with the highest enhancements in the green channel when applied by spray coating, and in the red and far-red channels when applied by sublimation coating. Matrix crystal size did not significantly impact the observed fluorescence enhancement effects for all six tested matrices. The two ionization modes of MALDI-1 and MALDI-2 imaging were also compared on brain, liver, and kidney tissue sections to assess lipid detection efficiency and spatial distribution in the FluoMALDI imaging pipeline. While the spatial distributions of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) species were consistent across MALDI ionization modes and matrix deposition methods, lipid detection efficiency varied depending on the matrix type, matrix crystal size, tissue type, and MALDI ionization mode. Sublimation, which produced fine crystals without solvents, generally resulted in higher phospholipid detection efficiency than spray coating for lipids including PE(36:2) and PC(36:2) in both MALDI-1 and MALDI-2 imaging. SIGNIFICANCE: These findings highlight that the matrix choice and matrix deposition method applied significantly affect tissue autofluorescence enhancement, spatial resolution, and lipid detection efficiency in MALDI-1 and MALDI-2 imaging modes, offering valuable insights for selecting optimal matrix deposition for FluoMALDI imaging based on a given study's research goals.

Indexed as

Optical ImagingSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationAnimalsBrainKidneyLiverMiceMicroscopy, FluorescenceAutofluorescenceFluoMALDIFluorescenceImagingMALDIMALDI-2MatrixMicroscopySprayingSublimation

Identifiers

PMID41073005
PMCPMC12838014

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.