Evidence mapPaperPMID 41089667Full record

ArticleLife medicine2025

Whole-process 3D ECM-encapsulated organoid-based automated high-throughput screening platform accelerates drug discovery for rare diseases.

Zhaoting Xu, Hui Yang, Yuru Zhou, Emmanuel Enoch Dzakah, Bing Zhao

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In one paragraph

Article in Life medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Zhaoting XuState Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China.ORCID https://orcid.org/0009-0004-3295-4869
Hui YangInstitute of Organoid Technology, Kunming Medical University, Kunming 650500, China.
Yuru ZhouSchool of Basic Medical Sciences, Institute of Biomedical Innovation, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330031, China.
Emmanuel Enoch DzakahZ Lab, bioGenous BIOTECH, Shanghai 200438, China.
Bing ZhaoInstitute of Organoid Technology, Kunming Medical University, Kunming 650500, China.ORCID https://orcid.org/0000-0001-9891-3569

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Organoid-based high-throughput screening (HTS) is revolutionizing pharmaceutical development. However, the complexity of handling extracellular matrix (ECM) components with traditional HTS devices leads to the use of suspension cultures for organoids during HTS, which alters their transcriptomic landscape and drug responses. Although automated generation techniques for 3D ECM-encapsulated organoids have been established, limitations in operational simplicity and time efficiency remain barriers to achieving high throughput. Here, we develop a whole-process 3D ECM-encapsulated organoid-based automated HTS (wp3D-OAHTS) platform, which achieves superior throughput compared to existing reported systems for 3D organoid drug screening. Utilizing this automated platform, we generated more than 10,000 homogeneous 3D organoid domes of neuroendocrine cervical cancer (NECC) and evaluated their drug responses to 2802 compounds in 13 days. This highly efficient and reproducible approach finally enabled the identification of 5 top hits that significantly inhibited NECC organoids

Indexed as

extracellular matrixhigh-throughput screeningneuroendocrine cervical cancerorganoidsQuisinostat 2HCl

Identifiers

PMID41089667
PMCPMC12517169

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.