Evidence map›Paper›PMID 41090210›Full record

ArticleImmunity, inflammation and disease2025

Investigating the Impact of the Secretome From hAMSCs on HT-29 Colon Cancer Cells via TNF-α/TGF-β/c-MYC Signaling Pathways Using a Three-Dimensional Cell Culture Model.

Mohammad Ali Majdoddin, Fatemeh Safari

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Article in Immunity, inflammation and disease, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Mohammad Ali MajdoddinDepartment of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
Fatemeh SafariDepartment of Biology, Faculty of Science, University of Guilan, Rasht, Iran.ORCID https://orcid.org/0000-0003-0300-7665

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundColon cancer is recognized as one of the predominant reasons of death globally. The ongoing cancer treatment techniques have not been successful; so, a strong and unique platform is needed. Interestingly, it has been discovered that stem cells offer a valuable and promising platform in cancer treatment. The goal of this study is to explore the influences of hAMSCs secretome on HT-29 colon cancer cells by analyzing TNF-α/TGF-βR/c-MYC signaling pathway, expression of interleukins (IL)-4/6/8, and cyclin-dependent kinase (CDK) inhibitory proteins (p15

methodsIn this study, a coculture system was employed. After 3 days, hAMSCs' secretome was collected and its effects on tumor formation, inflammation, and cell invasion were analyzed using Western blot, scratch assay, qPCR, and ELISA.

resultsThe study revealed a decrease in the TGF-βR/SMADs/c-MYC signaling pathway and an increase in levels of p15

conclusionsOur results could be useful in developing a new approach to treating cancer using hAMSCs' secretome.

Indexed as

Colonic NeoplasmsProto-Oncogene Proteins c-mycSecretomeSignal TransductionTransforming Growth Factor betaTumor Necrosis Factor-alphaCell Culture Techniques, Three DimensionalCoculture TechniquesHT29 CellsHumansMYC protein, humanProto-Oncogene Proteins c-mycTransforming Growth Factor betaTumor Necrosis Factor-alpha3D cell culture modelhAMSCsHT‐29 cancer cellsIL‐4/6/8 expressionTNF‐α/TGF‐βR/c‐MYC signaling pathways

Identifiers

PMID41090210
PMCPMC12521875

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.