Evidence map›Paper›PMID 41090362›Full record

ArticleThe Journal of clinical investigation2025

C6orf223 promotes colorectal cancer growth and metastasis by facilitating PRMT5-MEP50 multiprotein complex assembling.

Yufeng Qiao, Zhenzhen Wu, Peng Wang, Yiliang Jin, Furong Bai, Fei Zhang, Yunhe An, Meiying Xue, Han Feng, Yong Zhang and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of clinical investigation, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. CIITA/PRMT5 promote CD4BMC medicine · 2026
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Yufeng QiaoKey Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Zhenzhen WuKey Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Peng WangKey Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Yiliang JinCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Furong BaiKey Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Fei ZhangKey Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Yunhe AnInstitute of Analysis and Testing, Beijing Academy of Science and Technology (Beijing Center for Physical and Chemical Analysis), Beijing, China.
Meiying XueCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Han FengCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Yong ZhangKey Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Yaxin HouCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Junfeng DuDepartment of General Surgery, the Seventh Medical Center, Chinese PLA General Hospital, Beijing, China.
Huiyun CaiDepartment of General Surgery, the Seventh Medical Center, Chinese PLA General Hospital, Beijing, China.
Guizhi ShiUniversity of Chinese Academy of Sciences, Beijing, China.
Bing ZhouCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Pu GaoCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Jizhong LouKey Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Peng ZhangBeijing Key Laboratory for Genetics of Birth Defects, Beijing Pediatric Research Institute, MOE Key Laboratory of Major Diseases in Children, Rare Disease Center, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Kelong FanCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Jinbo LiuDepartment of Colorectal Surgery of the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Pengcheng BuKey Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein arginine methyltransferase 5 (PRMT5) complexes with methylosome protein 50 (MEP50) play crucial roles in tumor progress. However, the regulatory mechanism of governing the PRMT5-MEP50 hetero-octameric complex remains unclear. Here, we demonstrate that C6orf223, to our knowledge an uncharacterized protein, facilitates PRMT5-MEP50 multiprotein complex assembling, thereby promoting colorectal cancer (CRC) growth and metastasis. C6orf223 forms dimers through disulfide bonds, with its N-terminal arginine-enriched region binding to the C-terminal negatively charged groove of PRMT5, thus stabilizing PRMT5-MEP50 multiprotein and enhancing PRMT5 methyltransferase activity. Consequently, PRMT5-mediated H4R3me2s substantially decreases the expression of the tumor suppressor GATA5, leading to the upregulation of multiple oncogenic target genes including WWTR1, FGFR1, and CLU. Targeting C6orf223 using siRNAs encapsulated in ferritin protein shells effectively suppresses CRC tumor growth and metastasis. Collectively, our findings characterize the role of C6orf223 in facilitating PRMT5-MEP50 hetero-octameric complex assembling and suggest that C6orf223 could serve as a potential therapeutic target for CRC.

Indexed as

Adaptor Proteins, Signal TransducingColorectal NeoplasmsMultiprotein ComplexesNeoplasm ProteinsProtein-Arginine N-MethyltransferasesAnimalsCell Line, TumorCell ProliferationHCT116 CellsHumansMiceMice, NudeNeoplasm MetastasisProtein MultimerizationTranscription FactorsAdaptor Proteins, Signal TransducingMultiprotein ComplexesNeoplasm ProteinsPRMT5 protein, humanProtein-Arginine N-MethyltransferasesTranscription FactorsWDR77 protein, humanCell biologyColorectal cancerOncology

Identifiers

PMID41090362
PMCPMC12520688

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.