Evidence map›Paper›PMID 41104129›Full record

ArticleFrontiers in cellular and infection microbiology2025

Lele Pan, Lijian Wei, Shihua Luo, Baoyan Ren, Miao Li, Lina Liang, Xuebin Li, Guijiang Wei

Abstract read
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. CRISPR/Cas diagnostics forFrontiers in bioengineering and biotechnology · 2026
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Lele Pan *Center for Medical Laboratory Science, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China.
Lijian Wei *Center for Medical Laboratory Science, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China.
Shihua LuoCenter for Medical Laboratory Science, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China.
Baoyan RenYaneng BlOscience (Shenzhen) Corporation, Shenzhen, Guangdong, China.
Miao LiClinical Genome Center, Guangxi KingMed Diagnostics, Nanning, Guangxi, China.
Lina LiangCenter for Medical Laboratory Science, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China.
Xuebin LiBaise Key Laboratory for Precise Genetic Testing of Long-dwelling Nationalities, Baise, Guangxi, China.
Guijiang WeiCenter for Medical Laboratory Science, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Methods: This study developed a single-tube detection method combining recombinant polymerase amplification (RPA) and light-controlled CRISPR/Cas12a. RPA primers were designed and screened for the Results: The light-controlled RPA-CRISPR/Cas12a detection platform developed in this study uses a 15 μL reaction system. By optimizing key parameters such as RPA amplification time (20 min), primer concentration (400 nM), UV light activation time (30 s), and crRNA/Cas12a concentration (300 nM), the platform achieves optimal detection efficiency. The platform has a fluorescence detection limit of 4.072×10 Conclusion: We have successfully developed a light-controlled RPA-CRISPR/Cas12a detection system capable of rapidly and highly sensitively detecting

Indexed as

CRISPR-Cas SystemsKlebsiella InfectionsKlebsiella pneumoniaeNucleic Acid Amplification TechniquesBacterial ProteinsCRISPR-Associated ProteinsEndodeoxyribonucleasesHumansSensitivity and SpecificityUltraviolet RaysBacterial ProteinsCas12a proteinCRISPR-Associated ProteinsEndodeoxyribonucleasesCRISPR/Cas12aKlebsiella pneumoniaelight-controlledone-potrecombinase polymerase amplification

Identifiers

PMID41104129
PMCPMC12521164

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.