Evidence map›Paper›PMID 41105913›Full record

ArticleThe FEBS journal2026

Label-free imaging of intracellular structures in living mammalian cells via external apodization phase-contrast microscopy.

Hiroshi Ohno, Takenori Nishimura, Kenta Kainoh, Yoshitaka Ohashi, Naoko Onodera, Mayuko Kano, Lay Nurhana Sari, Masato Masuda, Yoshiaki Tamura, Teppei Nishino and 10 more

Abstract read
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In one paragraph

Article in The FEBS journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Hiroshi OhnoDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.
Takenori NishimuraDepartment of Animal Sciences and Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Kenta KainohDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.
Yoshitaka OhashiDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.
Naoko OnoderaDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.
Mayuko KanoDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.
Lay Nurhana SariDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.
Masato MasudaDepartment of Information Sciences and Arts, Faculty of Information Sciences and Arts, Toyo University, Saitama, Japan.
Yoshiaki TamuraDepartment of Information Sciences and Arts, Faculty of Information Sciences and Arts, Toyo University, Saitama, Japan.
Teppei NishinoDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.
Yusuke HayashiLaboratory of Integrative Oncology, National Cancer Center, Tokyo, Japan.
Yusuke YamamotoLaboratory of Integrative Oncology, National Cancer Center, Tokyo, Japan.ORCID 0000-0002-5262-8479
Shin-Ichiro TakahashiDepartment of Animal Sciences and Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Yuta MishimaTransborder Medical Research Center, University of Tsukuba, Japan.
Yosuke YoneyamaInstitute of Research, Tokyo Medical and Dental University, Tokyo, Japan.
Yoshinori TakeuchiDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.
Motohiro SekiyaDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.
Takashi MatsuzakaDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.
Takafumi MiyamotoDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.ORCID 0000-0001-9363-2864
Hitoshi ShimanoDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Japan.ORCID 0000-0002-5562-5572

Funding

Cabinet Office, Government of JapanCore Research for Evolutional Science and Technology JPMJCR1927Japan Science and Technology Agency JPMJPF2017Japan Society for the Promotion of Science KAKENHI (22H02296)Takeda Science Foundation DGM06005J
6 · The paper itself

Abstract

Developing techniques to visualize intracellular structures, which influence the spatiotemporal functionality of biomolecules, is essential for elucidating mechanisms governing cellular behavior. In this study, we demonstrate that label-free external apodized phase-contrast (ExAPC) microscopy serves as a valuable tool for the simultaneous observation of various intracellular structures with high spatiotemporal resolution, while successfully mitigating halo artifacts. Additionally, through quantitative analysis of images obtained by combining ExAPC microscopy with fluorescence microscopy, we identified distinct heterogeneities in biomolecular condensates, lipid droplets, and mitochondria. Our findings highlight the potential of ExAPC microscopy to provide detailed insights into alterations in intracellular structures associated with diverse cellular processes, corroborating the existing knowledge and potentially contributing to the discovery of previously unknown cellular mechanisms.

Indexed as

Lipid DropletsAnimalsBiomolecular CondensatesHeLa CellsHumansMicroscopy, FluorescenceMicroscopy, Phase-ContrastMitochondriabiomolecular condensatescellular organizationexternal apodization phase‐contrast microscopylipid dropletsmitochondria dynamics

Identifiers

PMID41105913
PMCPMC12914761

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.