Evidence map›Paper›PMID 41107455›Full record

ArticleCommunications biology2025

Decoding adult murine pancreatic islet cell diversity through cell type-resolved proteomics and phosphoproteomics.

Marvin Thielert, Adrian Villalba, Vincenth Brennsteiner, Maria Wahle, Constantin Ammar, Andreas-David Brunner, Alexis Fouque, Chloé Lourenço, Masaya Oshima, Latif Rachdi and 3 more

Abstract read
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Marvin Thielert *Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
Adrian Villalba *Institut Cochin, CNRS, Inserm, Université Paris Cité, Paris, France.
Vincenth BrennsteinerProteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
Maria WahleProteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.ORCID http://orcid.org/0000-0003-2079-4402
Constantin AmmarProteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
Andreas-David BrunnerProteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.ORCID http://orcid.org/0000-0002-2733-7899
Alexis FouqueInstitut Cochin, CNRS, Inserm, Université Paris Cité, Paris, France.
Chloé LourençoInstitut Cochin, CNRS, Inserm, Université Paris Cité, Paris, France.
Masaya OshimaInstitut Cochin, CNRS, Inserm, Université Paris Cité, Paris, France.
Latif RachdiInstitut Cochin, CNRS, Inserm, Université Paris Cité, Paris, France.ORCID http://orcid.org/0000-0002-1089-2837
Willem StaelsGenetics, Reproduction, and Development (GRAD), Beta Cell Neogenesis (BENE) Research Unit, Vrije Universiteit Brussel (VUB), Brussels, Belgium.ORCID http://orcid.org/0000-0001-8259-3329
Matthias MannProteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany. mmann@biochem.mpg.de.ORCID http://orcid.org/0000-0003-1292-4799
Raphaël ScharfmannInstitut Cochin, CNRS, Inserm, Université Paris Cité, Paris, France. raphael.scharfmann@inserm.fr.ORCID http://orcid.org/0000-0001-7619-337X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Islets of Langerhans are micro-organs scattered throughout the pancreas. They are composed of insulin-producing beta cells, glucagon-producing alpha cells, and somatostatin-producing delta cells. While their transcriptome has been extensively analyzed, protein-level information remains limited due to cell scarcity and purification challenges. Here, we combine cell sorting with highly sensitive mass spectrometry to create the first in-depth proteomic resource of pancreatic islet cells. We achieved a depth exceeding 6000 proteins per endocrine cell population, discovering new cell type-enriched ones. Deep proteomics profiling demonstrated that all three endocrine cell types were inflamed upon interferon gamma (IFNγ) treatment, a mediator of autoimmune damage in type 1 diabetes. Resolving the phosphoproteomic landscape of alpha, beta and delta cells with more than 7000 unique phosphosites per cell type provided insights into cell-specific signaling. This omics dataset offers a valuable resource for understanding pancreatic islet biology in health and disease.

Indexed as

Islets of LangerhansPhosphoproteinsProteomeProteomicsAnimalsGlucagon-Secreting CellsInsulin-Secreting CellsInterferon-gammaMiceMice, Inbred C57BLInterferon-gammaPhosphoproteinsProteome

Identifiers

PMID41107455
PMCPMC12534492

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.