Evidence map›Paper›PMID 41107668›Full record

ArticleIn vitro cellular & developmental biology. Animal2025

Cadmium-induced nucleus pulposus derived mesenchymal stem cells apoptosis via MAPK signaling pathway contributes to IVD degeneration.

Man Hu, Yu Zhang, Wen-Jie Zhao, Xin Liu, Yan-Feng Zhang, Da-Xu Liu, Xin-Min Feng, Liang Zhang

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Man Hu *Department of Orthopedic Surgery, Nanyang Second General Hospital, Nanyang, 473000, China.
Yu Zhang *Department of Orthopedics, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou, 225001, China.
Wen-Jie Zhao *Department of Orthopedics, Medical College of Nanjing University, Nanjing, 210000, China.
Xin LiuDepartment of Orthopedics, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou, 225001, China.
Yan-Feng ZhangDepartment of Orthopedic Surgery, Nanyang Second General Hospital, Nanyang, 473000, China.
Da-Xu LiuDepartment of Orthopedic Surgery, Nanyang Second General Hospital, Nanyang, 473000, China.
Xin-Min FengDepartment of Orthopedics, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou, 225001, China.
Liang ZhangDepartment of Orthopedics, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou, 225001, China. zhangliang6320@sina.com.

Funding

333 Project of Jiangsu Province (11) and Key Projects of Social development of Yangzhou City YZ2022091Cross Cooperation Project of Northern Jiangsu People's Hospital SBJC21014the National Natural Science Foundation of China 82172462the Traditional Chinese Medicine Science and Technology Development Plan Project of Jiangsu Province MS2021077
6 · The paper itself

Abstract

The current study aimed to explore the effect of Cadmium (Cd) on nucleus pulposus derived mesenchymal stem cells (NPMSCs) and the possible mechanism of IVDD caused by Cd. In this study, cell viability assay, EdU assay, TUNEL staining, flow cytometry assay, mRNA transcriptome sequencing, quantitative real-time polymerase chain reaction (PCR) assay, immunofluorescence assay and western blot assay were used to prove that Cadmium induces apoptosis of NPMSCs. Cd impaired the proliferation of NPMSCs and promoted cell apoptosis, and this effect was time and concentration dependent. Further study also found that the expression levels of senescence-related molecules (P16, P21 and P53) in the Cd group were up-regulated and the expression levels of pro-apoptotic molecules Bax and Caspase-3 in the Cd group were significantly up-regulated, while the expression level of anti-apoptotic molecule Bcl-2 was significantly down-regulated compared with those of the Control group. The MAPK signaling pathway-related proteins were detected, and the results found that the ratios of p-P38/P38 and p-JNK/JNK in the Cd group were significantly increased, while the ratios of p-ERK/ERK was significantly less compared with the control group, and it was in a concentration-dependent relationship. Cd can inhibit the activity and proliferation of NPMSCs in a dose and time-dependent manner, and promote cell aging and apoptosis. Cd may promote the apoptosis of NPMSCs by activating MAPK signaling pathway.

Indexed as

ApoptosisCadmiumIntervertebral Disc DegenerationMAP Kinase Signaling SystemMesenchymal Stem CellsNucleus PulposusAnimalsCell ProliferationCell SurvivalRatsRats, Sprague-DawleyCadmiumApoptosisCadmiumIntervertebral disc degenerationMAPK signaling pathwayNucleus pulposus derived mesenchymal stem cellPrevention

Identifiers

PMID41107668

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.