Evidence map›Paper›PMID 41108364›Full record

ArticleJournal of cancer research and clinical oncology2025

Running session-conditioned human serum lowers prostate cancer cell spheroid formation.

Giulia Baldelli, Alice Avancini, Diana Giannarelli, Lorenzo Budel, Veronica Gentilini, Anita Borsati, Linda Toniolo, Asja Conti, Michele Milella, Federico Schena and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cancer research and clinical oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Giulia BaldelliHygiene and Public Health Unit, Biomolecular Sciences Department, University of Urbino Carlo Bo, Urbino, PU, Italy.ORCID https://orcid.org/0000-0001-8254-2994
Alice AvanciniSection of Innovation Biomedicine-Oncology Area, Department of Engineering for Innovation Medicine (DIMI), University of Verona and University and Hospital Trust (AOUI) of Verona, P. le L.A. Scuro 10, 37134, Verona, Italy.ORCID https://orcid.org/0000-0003-2439-4799
Diana GiannarelliFacility of Epidemiology and Biostatistics, G-STeP, Fondazione Policlinico Universitario A. Gemelli, IRCCS, Rome, Italy.ORCID https://orcid.org/0000-0002-6085-1195
Lorenzo BudelDepartment of Neurosciences, Biomedicine and Movement, University of Verona, Verona, Italy.ORCID https://orcid.org/0009-0003-2103-1391
Veronica GentiliniHygiene and Public Health Unit, Biomolecular Sciences Department, University of Urbino Carlo Bo, Urbino, PU, Italy.ORCID https://orcid.org/0009-0006-3066-220X
Anita BorsatiDepartment of Neurosciences, Biomedicine and Movement, University of Verona, Verona, Italy.ORCID https://orcid.org/0000-0002-6898-091X
Linda TonioloDepartment of Neurosciences, Biomedicine and Movement, University of Verona, Verona, Italy.ORCID https://orcid.org/0009-0009-6842-3193
Asja ContiHygiene and Public Health Unit, Biomolecular Sciences Department, University of Urbino Carlo Bo, Urbino, PU, Italy.
Michele MilellaSection of Innovation Biomedicine-Oncology Area, Department of Engineering for Innovation Medicine (DIMI), University of Verona and University and Hospital Trust (AOUI) of Verona, P. le L.A. Scuro 10, 37134, Verona, Italy.ORCID https://orcid.org/0000-0002-3826-5237
Federico SchenaDepartment of Neurosciences, Biomedicine and Movement, University of Verona, Verona, Italy.ORCID https://orcid.org/0000-0002-2052-5117
Giorgio BrandiHygiene and Public Health Unit, Biomolecular Sciences Department, University of Urbino Carlo Bo, Urbino, PU, Italy.ORCID https://orcid.org/0000-0003-1380-6137
Sara PilottoSection of Innovation Biomedicine-Oncology Area, Department of Engineering for Innovation Medicine (DIMI), University of Verona and University and Hospital Trust (AOUI) of Verona, P. le L.A. Scuro 10, 37134, Verona, Italy. sara.pilotto@univr.it.ORCID https://orcid.org/0000-0003-2229-4874
Mauro De SantiHygiene and Public Health Unit, Biomolecular Sciences Department, University of Urbino Carlo Bo, Urbino, PU, Italy.ORCID https://orcid.org/0000-0003-2983-8344
Cantor TarperiDepartment of Neurosciences, Biomedicine and Movement, University of Verona, Verona, Italy.ORCID https://orcid.org/0000-0002-7208-9484

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposePhysical activity is associated with a lower mortality and recurrence risk in cancer, yet the underlying mechanisms remain unclear. This study aimed to evaluate the effects of running sessions on the tumorigenic potential of prostate cancer cells using a 3D in vitro model.

methodsFifteen healthy males completed two outdoor running sessions (5 km and 10 km), interspersed by 1 month of wash-out time. Blood samples were collected before (PRE), immediately after (POST), and 3 h after (POST-3 h) sessions. Human serum (HS) samples were used to stimulate LNCaP and PC3 cell lines in 3D in vitro culture technique. The spheroid formation ability was quantified after 21 days of incubation, using GelCount.

resultsIn both prostate cancer cell lines, a reduction in spheroid number was shown, by both running sessions and in all timepoints considered (LNCaP cells: 5 km: - 23.8%; 10 km: - 5.6% POST HS; 5 km: - 37.8%; 10 km: - 34.8% POST-3 h HS; PC3 cells: 5 km: - 14%; 10 km: - 15.9% POST HS; 5 km: - 14.2%; 10 km: - 13% POST-3 h HS). The spheroid volume was reduced by 42.6% (5 km) and 51.1% (10 km) with POST-3 h HS, in LNCaP cells; no significant reduction was observed in PC3 cells. No differences were found between the running sessions, while higher muscle mass, cardiorespiratory fitness and age were associated with greater reductions in spheroid number and volume, especially in LNCaP cells.

conclusionRunning sessions reduce prostate cancer cell spheroid formation, especially in participants with higher physical fitness. Shorter running distances showed comparable effects to longer ones, highlighting practical implications for real-world exercise prescriptions in oncology.

Indexed as

Prostatic NeoplasmsRunningSerumSpheroids, CellularAdultCell Line, TumorHumansMaleMiddle Aged3D cell cultureProstate cancerRunning sessionsSpheroid formationTertiary preventionTranslational research

Identifiers

PMID41108364
PMCPMC12535562

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.