Evidence map›Paper›PMID 41109915›Full record

ArticleBiologia futura2026

An optimized in situ hybridization protocol to study early developmental patterns and signaling pathways in paradise fish (Macropodus opercularis).

Nóra Szabó, Erika Fodor, Kata Szabó, Virág Tarcsa, Zsolt Gyulai, Soma Jakab, Erika Gelei, Dávid Czimer, Ádám Miklósi, Máté Varga

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Article in Biologia futura, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Nóra SzabóDepartment of Genetics, ELTE Eötvös Loránd University, Budapest, Hungary.
Erika FodorDepartment of Genetics, ELTE Eötvös Loránd University, Budapest, Hungary.
Kata SzabóDepartment of Genetics, ELTE Eötvös Loránd University, Budapest, Hungary.
Virág TarcsaDepartment of Genetics, ELTE Eötvös Loránd University, Budapest, Hungary.
Zsolt GyulaiDepartment of Genetics, ELTE Eötvös Loránd University, Budapest, Hungary.
Soma JakabDepartment of Genetics, ELTE Eötvös Loránd University, Budapest, Hungary.
Erika GeleiBabeș-Bolyai University, Cluj-Napoca, Romania.
Dávid CzimerDepartment of Genetics, ELTE Eötvös Loránd University, Budapest, Hungary.
Ádám MiklósiDepartment of Ethology, ELTE Eötvös Loránd University, Budapest, Hungary.
Máté VargaDepartment of Genetics, ELTE Eötvös Loránd University, Budapest, Hungary. mvarga@ttk.elte.hu.ORCID http://orcid.org/0000-0003-4289-1705

Funding

Magyar Tudományos Akadémia BO/00555/22/8Nemzeti Kutatási és Technológiai Hivatal 1783-3/2018/FEKUTSRAT
6 · The paper itself

Abstract

The Chinese paradise fish (Macropodus opercularis) is an obligate air-breathing species native to the hypoxic freshwater environments of Southeast Asia. While its ethological aspects have been extensively investigated since the 1970 s, the molecular biology of this species remains largely unexplored. Nevertheless, it offers significant potential for research in fields such as behavioral genetics, molecular evolution, and developmental biology. This study aims to optimize the in situ hybridization protocol, a widely used technique in developmental biology proven effective in zebrafish, and adapt it to paradise fish. We applied our optimized protocol to compare the expression of several conserved developmental genes, including chordin (chd), goosecoid (gsc), myogenic differentiation 1 (myod1), T box transcription factor Ta (tbxta), paired box 2a (pax2a), and retinal homebox gene 3 (rx3), in zebrafish and paradise fish embryos. Furthermore, we examined the role of key conserved signaling pathways during early development in both species using small molecular agonists and antagonists. Through these molecular analyses, we can gain deeper insights into the evolutionary conservation of early developmental programs.

Indexed as

FishesIn Situ HybridizationSignal TransductionAnimalsZebrafishEarly developmentGene expression patternsIn situ hybridizationParadise fishSignaling pathwaysZebrafish

Identifiers

PMID41109915

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.