ArticleMethods in molecular biology (Clifton, N.J.)2026
DNA Bioconjugation with Polymer Beads Using SNAP-tag® Technology.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
1 citing paper in PubMed.
- The SMC Hinge is a Selective Gate for Obstacle Bypass.Nature communications · 2025Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymer beads (such as Dynabeads) are commonly used to capture and isolate DNA and proteins, relevant for many applications in biochemistry and nanobiology. Current approaches to link DNA with beads involve non-covalent attachments such as biotin-streptavidin which pose a risk of spontaneous dissociation, potentially influencing data interpretation. We present a protocol to circumvent this risk by exploiting SNAP-tag technology to establish stable covalent anchoring (bioconjugate) between DNA and Dynabeads. We describe steps for generating benzylguanylated (BG) closed covalent circular DNA (BG-cccDNA), conjugating DNA with SNAP-tag and with beads, and enzymatic treatment and elution of conjugated DNA. We have successfully employed this technique to study the effect of Dynabeads as micrometer-scale DNA roadblocks on the motor activity of bacterial SMC Wadjet complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.