ArticleJournal of biomedical optics2025
Multiplane 2.5D microscopy for high-throughput high-resolution tissue imaging.
Article in Journal of biomedical optics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
4 authors.
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Abstract
Significance: Fast, high-throughput fluorescence imaging is essential for numerous biomedical applications, particularly in high-resolution volumetric tissue analysis. Aim: We aim to develop an imaging strategy that combines the strengths of multiplane microscopy and extended depth-of-field (EDOF) microscopy and to characterize its performance on tissue samples. Approach: We employed 2.5D microscopy, an EDOF approach optimized for high-resolution imaging, and integrated it with a quad-plane image splitter. This technique enables simultaneous capture of four focal volumes using a single camera, allowing volumetric imaging of Results: Our approach achieves a 25-fold reduction in image acquisition time compared with conventional Conclusions: This imaging technique provides a promising tool for tissue analysis, offering significant improvements in volumetric imaging speed with minimal compromise in spatial resolution and sensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.